SUMMARY: Sensitivity of conidia of Cochliobolus victoriae to fungistasis decreased markedly following incubation on moist sand for at least 1 h. Germination was greater on Conover loam or on sand being leached with water than on an alkaline clay loam soil known to produce a volatile fungistatic substance. Evolution of 14CO2 began within 3 min after [14C]glucose was applied to the soils; the rate of 14CO2 evolution was faster with Conover loam. Germinationot Thielaviopsis basicola conidia per unit of glucose remaining in agar discs initially containing 0·1% glucose, was lower for discs incubated on the clay loam soil than on Conover loam, and was greatest on a bed of sand undergoing aqueous leaching. Germination of ascospores of Neurospora tetrasperma and conidia of C. victoriae was suppressed on discs of washed, Purified Agar or polyacrylamide gel incubated on or over the clay loam soil, but no suppression resulted when discs were incubated on Conover loam. Extensive aeration of either soil did not remove its fungistatic effect. Fungistasis in Conover loam appears to be caused primarily by nutrient deprivation, whereas volatile fungistatic substances may play a major role in the clay loam soil.
AdamsP. B., LewisJ. A., PapavizasG. C.1968; Survival of root-infecting fungi in soil. IV. The nature of fungistasis in natural and cellulose-amended soil on chlamydospores of Fusarium solani f.sp. phaseoli. Phytopathology 58:378–383
BabiukL. A., PaulE. A.1970; The use of fluorescein isothiocyanate in the determination of the bacterial biomass of grassland soil. Canadian Journal of Microbiology 16:57–62
BishopD. H., ClaybrookJ. R., SpiegelmanS.1967; Electrophoretic separation of viral nucleic acids on polyacrylamide gels. Journal of Molecular Biology 36:373–387
BlakemanJ. P., FraserA. K.1971; Inhibition ofBotrytis cinerea spores by bacteria on the surface of chrysanthemum leaves. Physiological Plant Pathology 1:45–54
BrayG. A.1960; A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Annals of Biochemistry 1:279–285
BristowP. R., LockwoodJ. L.1975; Soil fungistasis: role of spore exudates in the inhibition of nutrient-independent propagules. Journal of General Microbiology 90:140–146
FarleyJ. D., LockwoodJ. L.1968; The suppression of actinomycetes by PCNB in culture media used for enumerating soil bacteria. Phytopathology 58:714–715
GrayT. R. G., WilliamsS. T.1971; Microbial productivity in soil. In Microbes and Biological Productivity, pp. 255–286HughesD. E. E., Rose.A. H. Edited by Cambridge University Press.;
JacksonR. M., KnightR. A.1973; Conidial germination ofCochliobolus sativus on soil and under artificial nutrient stress. Abstracts of Papers, Second International Congress of Plant Pathology Minneapolis: University of Minnesota;
LingappaB. T., LockwoodJ. L.1964; Activation of soil microflora by fungus spores in relation to soil fungistasis. Journal of General Microbiology 35:215–277