@article{mbs:/content/journal/micro/10.1099/00221287-90-1-13, author = "Sentandreu, R. and Elorza, M. Victoria and Villanueva, J. R.", title = "Synthesis of Yeast Wall Glucan", journal= "Microbiology", year = "1975", volume = "90", number = "1", pages = "13-20", doi = "https://doi.org/10.1099/00221287-90-1-13", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-90-1-13", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Summary:Saccharomyces cerevisiae was treated with a mixture of toluene and ethanol to make it permeable to small molecules. This treatment unmasked a glucan synthetase activity which was assayed with UDP-[U-14C]glucose. About 60% of the polymer formed was β(1→3)glucan. No labelled lipids were detected. The 14C incorporated was recovered in a particulate membrane preparation isolated by differential centrifugation. When the particles themselves were assayed for glucosyl transfer activity none was found. The toluene-treated preparations also catalysed the transfer of mannosyl residues from GDP-mannose to polymeric materials by a process independent of glucosyl transfer.", }