1887

Abstract

SUMMARY: To approach reproducibility of mass cultures as sources of enzymes, the growthrequirements of two mesophils belonging to and and three thermophils belonging to and were determined in metal-buffered media.

The mesophilic required glycerol or glucose, also alanine, aspartate, glycine, glutamate, arginine, histidine and lysine. Cytidylic acid or guanylic acid further increased growth; adenosine was inactive. The thermophilic required forgood growth either glycerol, gluconate, or glucose, also succinate or glutamate; glutamate was replaceable by the combination of arginine + histidine + proline. In glycerol media, either -malate alone, or citrate plus either acetate or fumarate supported growth; neither acetate nor fumarate were effective by themselves. In the absence of intermediatesof the citric acid cycle, sodium bicarbonate or a combination of protogen + acetate allowed growth. With acetate present, protogen was replaceable by thiamine.

The strain utilized glucose or sucrose; thiamine, biotin nicotnic acid were required. A combination of arginine + histidine + isoleucine increased growth. The carbohydrate requirement appeared absolute.

The thermophilic required glutamate or a combination of succinate + arginhe + histidine; these sufficed for moderate growth. Supplementation with glycerol, sucrose, gluconate or glucose increased growth. Further addition of acetate decreased the lag phase. For the mesophilic , either glucose or gluconate were suitable substrates; thiamine, biotin, riboflavin, and ‘reduced sulphur’ were required.

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1953-12-01
2022-01-17
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