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Volume 9, Issue 3

The Absorption of Polymyxin E by Bacteria and Bacterial Cell Walls and its Bactericidal Action Free

Abstract

SUMMARY: Isotherms of the absorption of polymyxin E by and after 20 min. contact with the antibiotic were determined. Tested on seven different bacteria, the maximum amounts of polymyxin E absorbed showed that the polymyxin-sensitive bacteria were capable of absorbing much greater quantities of the antibiotic than resistant organisms. When the bacteria were suspended in buffer solutions, the ultraviolet spectra of their supernatant fluids showeda maximum absorption at 260 m.; purines and pyrimidines contribute to this maximum. Addition of polymyxin E to suspensions of sensitive bacteria caused an immediate increase in the height of this maximum. Similar treatment of resistant organisms caused very little immediate increase in the amount of 260 m. absorbing material released. On prolonged incubation of untreated and polymyxin E treated cells there was a steady increase in the amount of 260 m. absorbing cell solutes released into the suspension media. Within the pH range 4·4–7·6 no optimum pH value was found for the polymyxin E absorption process or for the cellular release mechanism. The absorption isotherms of polymyxin E obtained with cell-wall preparations derived from sensitive and resistant bacteria showed that cell walk derived from sensitive organisms bound more polymyxin E than cell-wall preparations from resistant organisms. On treatment of sensitive bacteria with concentrations of polymyxin E less than those required for 99 % killing in 20 min. at 25°, there was a linear relationship between the amounts of 260 m. absorbing material released and the percentage of cells killed. Complete saturation of sensitive organisms with the antibiotic was not necessary to kill the cells. The amounts of polymyxh E. required for 99·5 % killing corresponded closely to those suflicient for the formation of a closely packed monolayer within the bacterial cell wall. It is suggested that, at these bactericidal concentrations, polymyxin E combines with and thereby disorganizes the structures responsible for the maintenance of osmotic equilibrium within the cell wall.

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© Society for General Microbiology, 1953
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1953-12-01
2024-04-25
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References

  1. Baker Z., Harrison R.W., Miller B.F. 1941; Inhibition by phospholipids of the action of synthetic detergents on bacteria.. J. exp. Med. 74:621
     [Google Scholar]
  2. Bliss E.A., Chandler C.A., Schoenbach E.B. 1949; In vitro studies of polymyxin.. Ann. N.Y. Acad. Sci. 51:944
     [Google Scholar]
  3. Brownlee G., Bushby S.R.M. 1948; Chemotherapy and pharmacology of aerosporin, a selective Gram-negative antibiotic.. Lancet i:127
     [Google Scholar]
  4. Brownlee G., Bushby S.R.M., Short E.I. 1952; The chemotherapy and pharmacology of the polymyxins.. Brit. J. Pharmacol. 7:170
     [Google Scholar]
  5. Dehéré CH. 1906; Sur l’absorption des rayons ultraviolets par l’acide nucleique extrait de la levure de biere.. C.R. Soc. Biol., Paris 60:34
     [Google Scholar]
  6. Few A.V., Schulman J.H. 1953a; Unimolecular films of polymyxins A, B, D and E at the air/water interface.. Biochem. J. 54:171
     [Google Scholar]
  7. Few A.V., Schulman J.H. 1953b; Absorption of polymyxin by bacteria.. Nature; Lond.: 171644
     [Google Scholar]
  8. Gale E.F., Taylor E.S. 1947; The assimilation of amino-acids by bacteria. 2. The action of tyrocidin and some detergent substance in releasing amino-acids from the internal environment of Streptococcus faecalis. . J. gen. Microbiol. 1:77
     [Google Scholar]
  9. Harkins W.D., Brown F.E. 1919; The determination of surface tension (free surface energy), and the weight of falling drops: the surface tension of water and benzene by the capillary height method.. J. Amer. chem. Soc. 41:499
     [Google Scholar]
  10. Hotchkiss R.D. 1946; The nature of the bactericidal action of surface active agents.. Ann. N.Y. Acad. Sci. 46:479
     [Google Scholar]
  11. Latterade C., Macheboeuf M. 1950; Recherches biochimiques sur le mode d’action de la polymyxine.. Ann. Inst.Pasteur 78:751
     [Google Scholar]
  12. Mitchell P., Moyle J. 1951; The glycerophospho-protein complex envelope of Micrococcus pyogenes. . J. gen. Microbiol. 5:981
     [Google Scholar]
  13. Pethica B.A., Schulman J.H. 1953; The physical chemistry of haemolysis by surface-active agents.. Biochem. J. 53:177
     [Google Scholar]
  14. Rybak B. 1950; Recherches sur la constitution du fuseau achromatique. 1. Ftudes chimiques de quelques modéles protéiques.. Bull. Soc. chim. Biol., Paris 32:703
     [Google Scholar]
  15. Salton M.R.J. 1951; The adsorption of cetyltrimethylammonium bromide by bacteria, its action in releasing cellular constituents and its bactericidal effects.. J. gen. Microbiol. 5:891
     [Google Scholar]
  16. Salton M.R.J. 1953; Studies on the bacterial cell wall. IV. The composition of the cell walls of some Gram-positive and Gram-negative bacteria.. Biochim. biophys.Acta 10:512
     [Google Scholar]
  17. White H.J., Alverson C.M., Baker M.J., Jackson E.R. 1949; Comparative biological studies of polymyxin and ‘aerosporin’.. Ann. N.Y. Acad. Sci. 51:879
     [Google Scholar]
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The Absorption of Polymyxin E by Bacteria and Bacterial Cell Walls and its Bactericidal Action
Microbiology 9, 454 (1953); https://doi.org/10.1099/00221287-9-3-454
/content/journal/micro/10.1099/00221287-9-3-454
/content/journal/micro/10.1099/00221287-9-3-454
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