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SUMMARY: Organisms may readily be isolated and cultured separately by micro-manipulation on the upper surface of an agar block. A micromanipulator is not essential, its place being taken by a second microscope with simple attachments. Illumination methods by dark-ground and phase-contrast techniques are described. A modification using a simple micromanipulator enables isolations to be made very rapidly. A device is described for making the necessary micro-instruments in a standard microscope.
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