1887

Abstract

SUMMARY: The paths of transfer of phosphate groups during uptake of inorganic orthophosphate by var. (strain Duncan) have been studied by following the incorporation of P-labelled phosphate groups into the phospholipid (LP), acid-soluble inorganic (AI) and organic (AO), deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and glycerophospho-protein complex (PGP) fractions of the cells.

In resting cells a strictly reciprocal exchange of inorganic phosphate across the osmotic barrier is half complete in 70 min. During respiration or growth, phosphate moves inward through the osmotic barrier at the same rate as during rest, but the outward movement is abolished. Phosphate accumulates mainly in the AI, AO and LP during respiration, but in all the fractions during growth. Most of the phosphate of the organic fractions is drawn through the AI. The RNA is formed from the nucleotides of the AO, while the DNA phosphate is derived either directly from the AI or from intermediates in the AO. Little or no turnover of RNA or DNA phosphate occurs during growth, and phosphate is not transferred directly or indirectly from RNA to DNA or vice versa. The PGP, although the major constituent of the mechanically rigid cell envelope, does not participate as a whole in phosphate transfer reactions during rest or respiration; but during growth the amount of PGP increases in proportion to the expansion of cell envelope area. The LP phosphate exhibits rapid turnover during respiration and growth.

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1953-10-01
2024-04-19
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References

  1. Boursnell , Coombs R.R., Rizk V. 1953; Studies with marked antisera. Biochem. J. In the press
    [Google Scholar]
  2. Brachet J. 1933; Recherches sur le synthèse de l’acide thymonucleique pendant le développement de l’œuf d’oursin. Arch. Biol., Paris 44:519
    [Google Scholar]
  3. Brues A.M., Tracey M.M., Cohn W.E. 1944; Nucleic acids of rat liver and hepatoma: their metabolic turnover in relation to growth. J. biol. Chem. 155:619
    [Google Scholar]
  4. Caldwell P.C. 1951; The nature of the phosphorus compounds present in cells of Bact. lactis aerogenes. . J. chem. Soc.166
    [Google Scholar]
  5. Caldwell P.C., Hinshelwood C. 1950; Some considerations on autosynthesis in bacteria. J. chem. Soc. p. 3156
    [Google Scholar]
  6. Caldwell P.C., Hinshelwood C. 1951; The phosphorus metabolism of Bact. lactis aerogenes. . J. chem. Soc. p. 158
    [Google Scholar]
  7. Caldwell P.C., Mackor E.L., Hinshelwood C. 1950; The ribose nucleic acid content and cell growth of Bact. lactis aerogenes. . J. chem. Soc. p. 3151
    [Google Scholar]
  8. Cohen S.S. 1951; Biochemical studies on multiplication of bacterial viruses. Fed. Proc. 10585
    [Google Scholar]
  9. Dische Z. 1930; Über einige neue characteristische Farbreaktionen der Thymo-nucleinsaure und eine Mikromethode zur Bestimmung derselben in tierischen Organen mit Hilfe dieser Reaktionen. Mikrochemie 8:4
    [Google Scholar]
  10. Fiske C.H., Subbarow Y. 1925; The colorimetric determination of phosphorus. J. biol. Chem. 66:375
    [Google Scholar]
  11. Furst S.S., Roll P.M., Brown G.B. 1950; On the renewal of the purines of the desoxypentose and pentose nucleic acids. J. biol. Chem. 183:251
    [Google Scholar]
  12. Juni E., Karen M.D., Reiner J.M., Spiegelman S. 1948; Turnover and dis-tribution of phosphate compounds in yeast metabolism. Arch. Biochem. 18:387
    [Google Scholar]
  13. Koch A.L., Putnam F.W., Evans E.A. 1952; Biochemical studies of virus reproduction. VIII. Purine metabolism. J. biol. Chem. 197:113
    [Google Scholar]
  14. Labaw L.W., Mosley V.M., Wyckoff R.W.G. 1950; Radioactive studies of the phosphorus metabolism of Escherichia coli. . J. Bact. 59:251
    [Google Scholar]
  15. Levy H.B., Skutch E.T., Schade A.L. 1949; Effect of cobalt on the phos�phorus turnover rate in the nucleic acids of Proteus vulgaris. . Arch. Biochem. 24:206
    [Google Scholar]
  16. Lundegårdh H. 1945; Absorption, transport and exudation of inorganic ions by the roots. Ark. Bot. 32 A 12:1
    [Google Scholar]
  17. Mitchell P. 1949; A new technique for stirred aerated culture. Nature; Lond.: 164846
    [Google Scholar]
  18. Mitchell P. 1953; Transport of phosphate across the surface of Micrococcus pyogenes: nature of the cell ‘inorganic phosphate’. J. gen. Microbiol. 9:273
    [Google Scholar]
  19. Mitchell P., Moyle J. 1951a; Relationships between cell growth, surface properties and nucleic acid production in normal and penicillin-treated Micrococcus pyogenes. . J. gen. Microbiol. 5:421
    [Google Scholar]
  20. Mitchell P., Moyle J. 1951b; The glycerophospho-protein complex envelope of Micrococcus pyogenes. . J. gen. Microbiol. 5:981
    [Google Scholar]
  21. Nelson V. 1944; A photometric adaptation of the somogyi method for the determination of glucose. J. biol. Chem. 153:375
    [Google Scholar]
  22. Roberts R.B., Roberts I.Z. 1950; Potassium metabolism in Escherichia coli. III. Interrelationship of potassium and phosphorus metabolism. J. cell. comp. Physiol. 36:15
    [Google Scholar]
  23. Schmidt G., Hecht L., Thannhauser S.J. 1948; The behaviour of the nucleic acids during the early development of the sea urchin egg. J. gen. Physiol. 31:203
    [Google Scholar]
  24. Schmidt G., Thannhauser S.J. 1945; A method for the determination of desoxyribonucleic acid, ribonucleic acid and phosphoproteins in animal tissues. J. biol. Chem. 161:83
    [Google Scholar]
  25. Schoenheimer R. 1942 The dynamic state of the body constituents. Harvard Univ. Press, Cambridge, Mass;
    [Google Scholar]
  26. Ussing H.H. 1947; Interpretation of the exchange of radio-sodium in isolated muscle. Nature; Lond.: 160:262
    [Google Scholar]
  27. Villee C.A., Lowens M., Gordon M., Leonard E., Rich A. 1949; The incorporation of 32P into the nucleoproteins and phosphoproteins of the developing sea urchin embryo. J. cell. comp. Physiol. 33:93
    [Google Scholar]
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