1887

Microbiology Society logo

Volume 9, Issue 1

Micromethod for the Methyl Red Test Free

Abstract

Summary: A micromethod for the methyl red (mr) test was worked out for suspensions of bacterial cells. The most important factors in the test are (1) the density of the cell suspension; (2) the ratio of glucose to buffer concentrations. Adaptation of the cells to glucose is essential for the success of the test, and cells from glucose-free media are not satisfactory. At the optimal glucose: buffer ratio and suspensions have different effects on the final pH value attained in the system.

Because the mr microtest is dependent on a fine balance between glucose and buffer concentrations, and is so greatly affected by even twofold differences in cell concentration, it is not really suitable for routine work in the characterization of bacteria.

  • Published Online:
© Society for General Microbiology, 1953
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-9-1-101
1953-08-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/9/1/mic-9-1-101.html?itemId=/content/journal/micro/10.1099/00221287-9-1-101&mimeType=html&fmt=ahah

References

  1. Clark W.M., Lubs H.A. 1915; The differentiation of bacteria of the eolon- aerogenes family by the use of indicators. J. infed. Dis. 17:160
     [Google Scholar]
  2. Clark W.M., Lubs H.A. 1917; Improved chemical methods for differentiating bacteria of the coli-aerogenes family. J. biol. Chem. 30:209
     [Google Scholar]
  3. Clarke P.H., Cowan S.T. 1952; Biochemical methods for bacteriology. J. gen. Microbiol. 6:187
     [Google Scholar]
  4. Cowan S.T. 1953; Fermentations: biochemical micromethods for bacteriology. J. gen. Microbiol. 8:391
     [Google Scholar]
  5. Harold C.H.H. 1936 Rep. metrop. Wat. Bd.30 pp 97–8
     [Google Scholar]
  6. Hoogerheide J.C. 1940; Studies on capsule formation. II. The influence of electrolytes on capsule formation by Klebsiella pneumoniae. . J. Bact. 39:649
     [Google Scholar]
  7. Report 1939; The bacteriological examination of water supplies. Rep. publ. Hlth med. Subj., Lond.71
     [Google Scholar]
  8. Richards T., Fouad M.T.A. 1952; The use of glucose inorganic media in the classification of coli-aerogenes bacteria. Proc. Soc. appl. Bact. 15 in the Press
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-9-1-101
Loading
Micromethod for the Methyl Red Test
Microbiology 9, 101 (1953); https://doi.org/10.1099/00221287-9-1-101
/content/journal/micro/10.1099/00221287-9-1-101
/content/journal/micro/10.1099/00221287-9-1-101
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error