1887

Abstract

SUMMARY: s6 sublethally heated at 52°C for 15 min in 0·1 -potassium phosphate buffer pH 7·2, lost neither the ribitol teichoic acid of the wall nor the glycerol teichoic acid of the membrane. Hurst . (1974) showed that this heating caused 40% loss of the cellular Mg, and we now report the loss of 65% of the ester-bound -alanine of teichoic acid. Repair from sublethal heat injury, measured by the return of salt tolerance, occurs in a simple no-growth medium provided that the cell concentration is < 5 × 10/ml. During repair, -alanine is rapidly synthesized. Fully-repaired cells contain four times more D-alanine than do freshly-injured cells. Magnesium is present in the medium at only 3 × 10 , yet the cellular Mg concentration returns to normal within 1 h of incubation, even in the presence of EDTA. The results suggest that repair occurs in two stages. Soon after injury, in the absence of the competitive effect of -alanine, Mg is strongly bound to teichoic acid. In repaired or uninjured cells Mg is less strongly bound. The implications of these findings are discussed in relation to the cation-binding function of teichoic acid.

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1975-08-01
2021-05-09
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