1887

Abstract

SUMMARY: The location of antigens on sections of bacteria using the soluble peroxidase-anti-peroxidase complex in conjunction with unlabelled antibody is described. Using this technique, spore antigens have been detected in the cytoplasm of vegetative cells during forespore septum formation and subsequent stages of sporulation. Antigenic sites were first associated with poly-beta-hydroxybutyric acid granules and subsequently were found in increasing quantities in the cytoplasm of the sporangium. Vegetative cell antigens were located on the cell wall and in the cortical region during sporulation. During germination antigens were located in the cortical region, and during outgrowth on the cell wall. These findings are discussed in the light of existing biochemical data.

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/content/journal/micro/10.1099/00221287-89-1-93
1975-07-01
2021-08-03
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References

  1. Aronson A. I., Fitz-James P. C. 1968; Biosynthesis of bacterial sporecoats. Journal of Molecular Biology 33:199–212
    [Google Scholar]
  2. Avrameas S. 1969; Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies. Immunochemistry 6:43–51
    [Google Scholar]
  3. Avrameas S., Uriel J. 1966; Méthode de marquage d’antigènes et d’anticorps avec des enzymes et son application en immunodiffusion. Comptes rendus hebdomadaire des séances de l’Academie de Sciences 262:2543–2545
    [Google Scholar]
  4. Baillie A., Thomson R. O., Batty I., Walker P. D. 1967; Some preliminary observations on the location of esterases in Bacillus cereus. Journal of Applied Bacteriology 30:312–316
    [Google Scholar]
  5. Hamilton W. A., Stubbs J. M. 1967; Comparison of the germination and outgrowth of spores of Bacillus cereus and Bacillus polymyxa. Journal of General Microbiology 47:121–129
    [Google Scholar]
  6. Hämmerling U., Aoki T., Harven E., Coyse E. A., Old L. J. 1968; Use of hybrid antibodies with anti-yG and antiferritin specificities in locating cell surface antigens by electron microscopy. Journal of Experimental Medicine 128:1461–1469
    [Google Scholar]
  7. Hanson R. S., Peterson J. A., Yousten A. A. 1970; Unique biochemical events in bacterial sporulation. Annual Review of Microbiology 24:53–90
    [Google Scholar]
  8. Hinton D. M., Petrali J. P., Meyer H. G., Sternberger L. A. 1973; The unlabelled antibody enzyme method of immunohistochemistry. Molecular immunocytochemistry of antibodies on the erythrocyte surface. Journal of Histochemistry and Cytochemistry 21:978–998
    [Google Scholar]
  9. Kawarai Y., Nakane P. K. 1970; Localisation of tissue antigens on ultrathin sections with peroxidase-labelled antibody method. Journal of Histochemistry and Cytochemistry 18:161–166
    [Google Scholar]
  10. Mason T. E., Phifer R. F., Spicer S. S., Swallow R. A., Dreskin R. B. 1969; An immunoglobulin- enzyme bridge method for localising tissue antigens. Journal of Histochemistry and Cytochemistry 17:563–569
    [Google Scholar]
  11. Moriarty G. C., Halmi N. S. 1972; Electron microscopic study of the adrenocorticotropin producing cell with the use of unlabelled antibody and the soluble peroxidase-anti-peroxidase complex. Journal of Histochemistry and Cytochemistry 20:590–603
    [Google Scholar]
  12. Moriarty G. C., Moriarty M., Sternberger L. A. 1973; Ultrastructural immunocytochemistry with unlabelled antibodies and the peroxidase-anti-peroxidase complex. A technique more sensitive than radioimmunoassay. Journal of Histochemistry and Cytochemistry 21:825–833
    [Google Scholar]
  13. Murrell W. G., Ohye D. F., Gordon R. A. 1969; Cytological and chemical structure of the spore. In Spores 4 pp. 1–19 Campbell. L. L. Edited by Washington: American Society for Microbiology.;
    [Google Scholar]
  14. Nakane P., Pierce G. 1967a; Enzyme labelled antibodies preparation and application for the localisation of antigens. Journal of Histochemistry and Cytochemistry 14:929–931
    [Google Scholar]
  15. Nakane P., Perce G. 1967b; Enzyme labelled antibodies for the light and electron microscopic localisation of tissue antigens. Journal of Cell Biology 33:307–310
    [Google Scholar]
  16. Ohye D., Murrell W. G. 1962; Formation and structure of the spore of Bacillus coagulans. Journal of Cell Biology 14:111–123
    [Google Scholar]
  17. Ohye D., Murrell W. G. 1973; Exosporium and sporecoat formation in Bacillus cereus. Journal of Bacteriology 115:1179–1190
    [Google Scholar]
  18. Petrali J. P., Hinton D. M., Moriarty G. C., Sternberger L. A. 1974; The unlabelled antibody enzyme method of immunohistochemistry. Quantitative comparison of sensitivities with and without peroxidase-anti-peroxidase complex. Journal of Histochemistry and Cytochemistry 22:782–801
    [Google Scholar]
  19. Sternberger L. A. 1974 Immunocytochemistry. Englewood Cliffs, New Jersey: Prentice-Hall.;
    [Google Scholar]
  20. Sternberger L. A., Cuculis J. J. 1969; Method for enzymatic intensification of the immunocyto-chemical reaction without the use of labelled antibodies. Journal of Histochemistry and Cytochemistry 17:190
    [Google Scholar]
  21. Sternberger L. A., Hardy P. H.Jun. Cuculis J. J., Meyer H. G. 1970; The unlabelled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. Journal of Histochemistry and Cytochemistry 18:315–333
    [Google Scholar]
  22. Tipper D. J., Gauthier J. J. 1972; Structure of the bacterial endospore. In Spores 5 pp. 3–12 Halvorson H.O, Hanson R., Campbell L.L. Edited by Washington: American Society for Microbiology.;
    [Google Scholar]
  23. Walker P. D. 1969; The location of chemical components on ultrathin sections of Bacillus cereus embedded in glycol methacrylate. Journal of Applied Bacteriology 32:463–467
    [Google Scholar]
  24. Walker P. D., Baillie A., Thomson R. O., Batty I. 1966; The use of ferritin labelled antibodies in the location of spore and vegetative antigens of Bacillus cereus. Journal of Applied Bacteriology 29:512–518
    [Google Scholar]
  25. Walker P. D., Batty I. 1964; Fluorescent studies in the genus Clostridium. I. The location of antigens on the surface of Clostridium sporogenes during sporulation and germination. Journal of Applied Bacteriology 27:137–139
    [Google Scholar]
  26. Walker P. D., Batty I. 1965; Surface antigenic changes in Bacillus cereus during germination and sporulation as shown by fluorescent staining. Journal of Applied Bacteriology 28:194–196
    [Google Scholar]
  27. Walker P. D., Thomson R. O. 1972; Immunology of spores and sporeforms. In Spores 5 pp. 321–337 Halvorson H.O, Hanson R., Campbell L. L. Edited by Washington: American Society for Microbiology.;
    [Google Scholar]
  28. Walker P. D., Thomson R. O., Baillie A. 1967; Use of ferritin labelled antibodies in the location of spore and vegetative antigens of Bacillus subtilis. Journal of Applied Bacteriology 30:317–320
    [Google Scholar]
  29. Walker P. D., Thomson R. O., Short J. A. 1971; Location of chemical components on ultrathin sections of bacteria. Spore Research 1971 pp. 202–209 Barker A. N., Gould G. W., Wolf J. Edited by London: Academic Press.;
    [Google Scholar]
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