1887

Microbiology Society logo

Volume 89, Issue 1

A Kinetic Study of a Solubilized Chitin Synthetase Preparation from Free

Abstract

SUMMARY: The solubilized chitin synthetase preparation from the stipes of is activated by its substrate, UDP--acetylglucosamine, and by carbohydrates, in particular -acetylglucosamine, diacetylchitobiose and glucose. Analysis of the results of variation in enzyme activity with UDP-GlcNAc concentration by several methods suggested that this substrate is an allosteric activator, with Hill coefficients close to 2 and 4 at concentrations above and below 0·1 m respectively, and an [S] value of 0·9 m. -acetylglucosamine lessened but did not abolish the sigmoidal shape of the velocity-substrate concentration plot. The chitin synthetase was inhibited by adenine and uridine nucleotides, and uridine diphosphate was a powerful competitive inhibitor. The enzyme preparation also contained a nucleoside diphosphatase activity and the implications of the removal of the inhibitory UDP formed by the chitin synthetase are considered.

  • Received:
  • Revised:
  • Published Online:
© Society for General Microbiology, 1975
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-89-1-146
1975-07-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/89/1/mic-89-1-146.html?itemId=/content/journal/micro/10.1099/00221287-89-1-146&mimeType=html&fmt=ahah

References

  1. Atkinson D. E., Hathaway J. A., Smith E. C. 1965; Kinetics of regulatory enzymes. Kinetic order of the yeast diphosphopyridine nucleotide isocitrate dehydrogenase reaction and a model for the reaction. Journal of Biological Chemistry 240:2682–2690
     [Google Scholar]
  2. Camargo E. P., Dietrich C. P., Sonneborn D., Strominger J. L. 1967; Biosynthesis of chitin in the spores and growing cells of Blastocladiella emersonii. Journal of Biological Chemistry 242:3121–3128
     [Google Scholar]
  3. Fajszi C., Endrenyi L. 1974; New linear plots for the separate estimation of Michaelis-Menten parameters. FEBS Letters 44:240–246
     [Google Scholar]
  4. Gooday G. W. 1972a; The effect of Polyoxin D on morphogenesis in Coprinus cinereus. Biochemical Journal 129:17–18 p
    [Google Scholar]
  5. Gooday G. W. 1972b; The role of chitin synthetase in the elongation of fruit bodies of Coprinus cinereus. Journal of General Microbiology 73:xxi
     [Google Scholar]
  6. Gooday G. W. 1973; Activity of chitin synthetase during the development of fruit bodies of Coprinus cinereus. Biochemical Society Transactions 1:1105–1107
     [Google Scholar]
  7. Gooday G. W., de Rousset-Hall A. 1975; Properties of chitin synthetase from Coprinus cinereus. Journal of General Microbiology 89:137–145
     [Google Scholar]
  8. Kent P. W., Ackers J. P., White R. J. 1970; N-iodoacetyl-d-glucosamine, an inhibitor of growth and glycoside uptake in Escherichia coli. Biochemical Journal 118:73–79
     [Google Scholar]
  9. Kent P. W., Dwek R. A. 1971; N-fluoroacetyl-α-D-glucosamine as a molecular probe of lysozyme structure by using [19F]fluorine nuclear-magnetic-resonance techniques. Biochemical Journal 121:11–12 p
    [Google Scholar]
  10. McMurrough I., Bartnicki-Garcia S. 1971; Properties of a particulate chitin synthetase from Mucor rouxii. Journal of Biological Chemistry 246:4008–4016
     [Google Scholar]
  11. Monod J., Changeux J. P., Jacob F. 1963; Allosteric proteins and cellular control systems. Journal of Molecular Biology 6:306–329
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-89-1-146
Loading
A Kinetic Study of a Solubilized Chitin Synthetase Preparation from Coprinus cinereus
Microbiology 89, 146 (1975); https://doi.org/10.1099/00221287-89-1-146
/content/journal/micro/10.1099/00221287-89-1-146
/content/journal/micro/10.1099/00221287-89-1-146
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error