Properties of Chitin Synthetase from Free

Abstract

SUMMARY: Preparations of chitin synthetase (EC. 2.4.1.16) from the stipe of the toadstool were characterized. Microsomal fractions had a very high specific activity. The chitin synthetase had been solubilized by treatment with digitonin, with an increase in specific activity and in stability. Optimum conditions for enzyme activity were determined. These preparations needed only a divalent cation and UDP--acetylglucosamine for chitin synthesis and showed no primer requirement. The sole glycosyl product was characterized as macro-molecular chitin.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-89-1-137
1975-07-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/89/1/mic-89-1-137.html?itemId=/content/journal/micro/10.1099/00221287-89-1-137&mimeType=html&fmt=ahah

References

  1. Flowers H. M., Batra K. K., Kemp J., Hassid W. Z. 1968; Biosynthesis of insoluble glucans from Phaseolus aureus and Lupinus albus. Plant Physiology 43:1703–1709
    [Google Scholar]
  2. Glaser L., Brown D. H. 1957; The synthesis of chitin in cell-free extracts of Neurospora crassa. Journal of Biological Chemistry 228:729–742
    [Google Scholar]
  3. Gooday G. W. 1972a; The effect of polyoxin D on morphogenesis in Coprinus cinereus. Biochemical Journal 129:17–18 p
    [Google Scholar]
  4. Gooday G. W. 1972b; The role of chitin synthetase in the elongation of fruit bodies of Coprinus cinereus. Journal of General Microbiology 73:xxi
    [Google Scholar]
  5. Gooday G. W. 1973; Activity of chitin synthetase during the development of fruit bodies of the toadstool Coprinus cinereus. Biochemical Society Transactions 1:1105–1107
    [Google Scholar]
  6. Gooday G. W. 1974; Control of development of excised fruit bodies and stipes of Coprinus cinereus. Transactions of the British Mycological Society 62:391–399
    [Google Scholar]
  7. Jaworski E. G., Wang L. C., Carpenter W. D. 1965; Biosynthesis of chitin in cell-free extracts of Venturia inaequalis. Phytopathology 55:1309–1312
    [Google Scholar]
  8. Kemp J., Loughman B. C. 1974; Cyclitol glucosides and their role in the synthesis of a glucan from uridine diphosphate glucose in Phaseolus aureus. Biochemical Journal 142:153–159
    [Google Scholar]
  9. Lowry D. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  10. Mcmurrough I., Bartnicki-Garcia S. 1971; Properties of a particulate chitin synthetase from Mucor rouxii. Journal of Biological Chemistry 246:4008–4016
    [Google Scholar]
  11. Porter C. A., Jaworski E. G. 1966; The synthesis of chitin by particulate preparations of Allomyces macrogynus. Biochemistry 5:1149–1154
    [Google Scholar]
  12. De Rousset-Hall A., Gooday G. W. 1975; A kinetic study of a solubilized chitin synthetase preparation from Coprinus cinereus. Journal of General Microbiology 89:146–154
    [Google Scholar]
  13. Ruiz-Herrera J., Bartnicki-Garcia S. 1974; Synthesis of cell wall microfibrils in vitro by a soluble chitin synthetase from Mucro rouxii. Science; New York: 186357–359
    [Google Scholar]
  14. Zechmeister L., Toth G. 1931; Zur Kenntnis der Hydrolyse von Chitin mit Salzsäure. Berichte der Deutschen chemischen Gesellschaft 64:2028–2031
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-89-1-137
Loading
/content/journal/micro/10.1099/00221287-89-1-137
Loading

Data & Media loading...

Most cited Most Cited RSS feed