@article{mbs:/content/journal/micro/10.1099/00221287-89-1-11, author = "Sim, I. S. and Stephen, J.", title = "Studies on the Purification of Chlamydial Agents Grown in Yolk Sacs of Embryonated Eggs Using Disulphide-linked Immunosorbents and Enzymes", journal= "Microbiology", year = "1975", volume = "89", number = "1", pages = "11-25", doi = "https://doi.org/10.1099/00221287-89-1-11", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-89-1-11", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: Immunosorbents were derived from avid and non-avid sera raised in rabbits to multiple or single injections of chlamydiae passaged once or three times in HeLa cells after routine passage in eggs. Egg-derived suspensions of chlamydiae required pretreatment before application to immunosorbent columns; this was most conveniently done by fractionation on Sepharose 4B. Immunosorbents derived from avid serum had greater capacity than those from non-avid sera. However, organisms were desorbed in low yield from an avid immunosorbent, but in higher yields from non-avid immunosorbents. Even under the best conditions, the immunosorbents yielded suspensions of organisms still contaminated with egg antigens. Partially purified suspensions of chlamydiae were also treated with phospholipase C to yield suspensions of high purity.", }