%0 Journal Article %A Eggerding, Caroline %A Randall, Judith A. %A Sargent, M. L. %T An Altered Invertase in the cot-2 Mutant of Neurospora crassa %D 1975 %J Microbiology, %V 89 %N 1 %P 102-112 %@ 1465-2080 %R https://doi.org/10.1099/00221287-89-1-102 %I Microbiology Society, %X SUMMARY: Because the cot-2 and inv loci of Neurospora crassa are closely linked, the invertase from the morphological mutant, cot-2, was examined. The cot-2 strains produce an invertase with altered heat sensitivity, K m, and ratio of heavy to light forms. The cellular localization of cot-2 invertase is different from that of the wild type. There were no observable changes in the energy of activation or the pH optimum of cot-2 invertase, and some of the differences detected were not apparent under culture conditions that promoted wild-type growth. Since recombination (about 5 %) occurred between cot-2 and inv and culture conditions affected the enzyme characteristics, we suggest cot-2 determines, in part, the carbohydrate composition of the enzyme. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-89-1-102