1887

Abstract

SUMMARY: Two strains of heterotrophic, non-motile, Gram-negative extreme thermophiles have been isolated from hot tap water. These strains (NH and DI) have been characterized and compared with strains of the genus Few of the single organic compounds tested supported growth in the presence of ammonium salts, and, like Thermus strains, growth on undefined media was restricted to dilute tryptone-yeast extract-mineral salt solutions. Nutrient agar and similar common laboratory media did not support growth. The growth rate was similar to that of Thermus strains, as was the unusual pattern of antibiotic resistance. Mean base composition of NH was 61·4% guanine plus cytosine (G + C), and of DI was 62·0 to 62·2% G + C, which are several per cent lower than the G + C contents of other strains so far described.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-88-2-321
1975-06-01
2021-08-01
Loading full text...

Full text loading...

/deliver/fulltext/micro/88/2/mic-88-2-321.html?itemId=/content/journal/micro/10.1099/00221287-88-2-321&mimeType=html&fmt=ahah

References

  1. Brock T. D., Boylen K. L. 1973; Presence of thermophilic bacteria in laundry and domestic hot-water heaters. Applied Microbiology 25:72–76
    [Google Scholar]
  2. Brock T. D., Freeze H. 1969; Thermus aquaticus gen.n. and sp.n., a non-sporulating extreme thermophile. Journal of Bacteriology 98:289–297
    [Google Scholar]
  3. Jackson T. J., Ramaley R. F., Meinschein W. G. 1973; Thermomicrobium, a new genus of extremely thermophilic bacteria. International Journal of Systematic Bacteriology 23:28–36
    [Google Scholar]
  4. Heinen U. J., Klein G., Klein H. P., Heinen W. 1971; Comparative studies on the nature and distribution of pigments from two thermophilic bacteria. Archiv für Mikrobiologie 76:18–27
    [Google Scholar]
  5. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  6. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. Journal of Molecular Biology 5:109–118
    [Google Scholar]
  7. Mcfeters G. A., Ulrich J. T. 1972; Effect of temperature on the respiration and cytochromes of an extreme thermophile. Journal of Bacteriology 110:777–779
    [Google Scholar]
  8. Oshima T. 1972; Studies on an extreme thermophile, Flavobacterium thermophilum HB8. In Molecular Evolution: Prebiological and Biological pp. 399–423 Edited by Rohlfing D. L., Oparin A. L. New York: Plenum Press;
    [Google Scholar]
  9. Oshima T., Imahori K. 1971; Isolation of an extreme thermophile and thermostability of its transfer ribonucleic acid and ribosomes. Journal of General and Applied Microbiology 17:513–517
    [Google Scholar]
  10. Oshima T., Imahori K. 1974; Description of Thermus thermophiles(Yoshida arid Oshima) Comb. Nov. A non-sporulating thermophilic bacterium from a Japanese thermal spa. International Journal of Systematic Bacteriology 24:102–112
    [Google Scholar]
  11. Ramaley R. F., Hixon J. 1970; Isolation of a non-pigmented, thermophilic bacterium similar to Thermus aquaticus. . Journal of Bacteriology 103:527–528
    [Google Scholar]
  12. Schildkraut C., Lifson S. 1965; Dependence of the melting temperatures of DNA on salt concentration. Biopolymers 3:195–208
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-88-2-321
Loading
/content/journal/micro/10.1099/00221287-88-2-321
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error