1887

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Volume 88, Issue 2

Specialized Transduction of Kanamycin Resistance in a Providence Strain Free

Abstract

SUMMARY: Properties of a transducing system with a phage able to transduce a kanamycin-resistance marker of the T compatibility group plasmid R394 at a frequency of 2 × 10/plaque-forming unit adsorbed are described. The phage was detected in Providence strain P29 transduced to kanamycin resistance by Providence phage PL25 grown on this strain harbouring the R factor. Four P29 transductants, specially selected at the lowest multiplicities of infection of the high frequency transducing (HFT) phage, were defective lysogens. They plated PL25 with an efficiency of 1 and only one liberated low-titre phage spontaneously or on u.v. induction. The defect in maturation function could be corrected by introduction of a wild PL25 prophage. The transducing phage was serologically identical to PL25. It could transduce in single infection, but transduction frequency was increased by the simultaneous presence of homologous non-transducing phage. Transductants did not transfer the kanamycin-resistance marker by conjugation, and produced kanamycin-sensitive segregants at a moderate rate. These segregants could be transduced to kanamycin resistance by the HFT phage. Irradiation of HFT lysates by u.v. produced an exponential fall in transduction frequency. It was concluded that the defective phage transduced by lysogenization. Kanamycin-resistant transductants could themselves be transduced to streptomycin resistance by PL25 reared on a streptomycin-resistant mutant. Lysogenic transductants produced by the HFT phage did not always liberate HFT phage on u.v. induction. Possible explanations are considered.

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© Society for General Microbiology 1975
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1975-06-01
2024-04-16
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References

  1. Adams M. H. 1956; Methods of study of bacterial viruses. Methods in Medical Research 2:1–73
     [Google Scholar]
  2. Arber W. 1958; Transduction des caractères gal par le bactèriophage lambda. Archives scientiae (Geneva) 11:259–338
     [Google Scholar]
  3. Arber W. 1960; Transduction of chromosomal genes and episomes in Escherichia coli. Virology 11:273–288
     [Google Scholar]
  4. Adler J., Templeton B. 1963; The amount of galactose genetic material in Adg bacteriophage with different densities. Journal of Molecular Biology 7:710–720
     [Google Scholar]
  5. Backhaus H., Schmieger H. 1974; The origin of the DNA in a special class of generalized transducing particles of Salmonella- phage P22. Molecular and General Genetics 131:123–135
     [Google Scholar]
  6. Bernstein A., Rolfe B., Onodera K. 1973; The E. coli cell surface: isolation of A transducing phages carrying the ta/PAB cluster. Molecular and General Genetics 121:325–335
     [Google Scholar]
  7. Campbell A. 1957; Transduction and segregation in Escherichia coli K12. Virology 4:366–384
     [Google Scholar]
  8. Chan R. K., Botstein D., Watanabe T., Ogata Y. 1972; Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. Virology 50:883–898
     [Google Scholar]
  9. Clowes R. C., Hayes W. 1968 Experiments in Microbial Genetics Oxford and Edinburgh: Blackwell Scientific Publications;
     [Google Scholar]
  10. Coetzee J. N. 1972; Genetics of the Proteus group. Annual Review of Microbiology 26:23–54
     [Google Scholar]
  11. Coetzee J. N. 1974a; Properties of Proteus and Providence strains harbouring recombinant plasmids between P-lac and R1drd 19 or R447F. Journal of General Microbiology 80:119–130
     [Google Scholar]
  12. Coetzee J. N. 1974b; High frequency transduction of kanamycin resistance in Proteus mirabilis. Journal of General Microbiology 84:285–296
     [Google Scholar]
  13. Coetzee J. N. 1975; High frequency transduction of ampicillin and kanamycin resistance in Proteus mirabilis. Journal of General Microbiology 87:173–176
     [Google Scholar]
  14. Coetzee J. N., Datta N., Hedges R. W. 1972; R factors from Proteus rettgeri. Journal of General Microbiology 72:543–552
     [Google Scholar]
  15. Coetzee J. N., Datta N., Hedges R. W., Appelbaum P. C. 1973; Transduction of R factors in Proteus mirabilis and P. rettgeri. Journal of General Microbiology 76:355–368
     [Google Scholar]
  16. Coetzee J. N., Sacks T. G. 1960; Transduction of streptomycin resistance in Proteus mirabilis. Journal of General Microbiology 23:445–455
     [Google Scholar]
  17. Coetzee J. N., Smit J. A., Prozesky O. W. 1966; Properties of Providence and Proteus morganii transducing phages. Journal of General Microbiology 44:167–176
     [Google Scholar]
  18. Dubnau E., Stocker B. A. D. 1964; Genetics of plasmids in Salmonella typhimurium. Nature; London: 204:1112–1113
     [Google Scholar]
  19. Gratia J. P. 1973; Coliphage ɸy, a novel type of specialized transducer. Molecular and General Genetics 124:157–166
     [Google Scholar]
  20. Hedges R. W., Jacob A. E. 1974; Transposition of ampicillin resistance from RP4 to other replicons. Molecular and General Genetics 132:31–40
     [Google Scholar]
  21. Hoppe I., Roth J. 1974; Specialized transducing phages derived from Salmonella phage P22. Genetics 76:633–654
     [Google Scholar]
  22. Jessop A. P. 1972; A specialized transducing phage of P22 for which the ability to form plaques is associated with transduction of the pro MS region. Molecular and General Genetics 114:214–222
     [Google Scholar]
  23. Kameda M., Harada K., Suzuki M., Mitsuhashi S. 1965; Drug resistance of enteric bacteria. V. High frequency of transduction of R factors with bacteriophage epsilon. Journal of Bacteriology 90:1174–1181
     [Google Scholar]
  24. Kondo E., Mitsuhashi S. 1964; Drug resistance of enteric bacteria. IV. Active transducing bacteriophage Pi CM produced by the combination of R factor with bacteriophage P1. Journal of Bacteriology 88:1266–1276
     [Google Scholar]
  25. Krizsanovich K. 1973; Cryptic lysogeny in Proteus mirabilis. Journal of General Virology 19:311–320
     [Google Scholar]
  26. Lederberg J. 1950; Isolation and characterization of biochemical mutants of bacteria. Methods in Medical Research 3:5–18
     [Google Scholar]
  27. Luria S. E., Adams J. N., Ting R. C. 1960; Transduction of lactose-utilizing ability among strains of E. coli and S. dysenteriae and the properties of the transducing phage particles. Virology 12:348–390
     [Google Scholar]
  28. Matsushiro A. 1963; Specialized transduction of tryptophan markers in Escherichia coli K12 by bacteriophage ɸ 80. Virology 19:475–482
     [Google Scholar]
  29. Ozeki H., Ikeda H. 1968; Transduction mechanisms. Annual Review of Genetics 2:245–278
     [Google Scholar]
  30. Prozesky O. W., Grabow W. O. K., Van Der Merwe S., Coetzee J. N. 1973; Arginine gene clusters in the Proteus-Providence group. Journal of General Microbiology 77:237–240
     [Google Scholar]
  31. Rae M. E., Stodolsky M. 1974; Chromosome breakage, fusion and reconstruction during P1dl transduction. Virology 58:32–54
     [Google Scholar]
  32. Schmieger H. 1972; Phage P22 mutants with increased or decreased transduction abilities. Molecular and General Genetics 119:75–88
     [Google Scholar]
  33. Smith-Keary P. F. 1966; Restricted transduction by bacteriophage P22 in Salmonella typhimurium. Genetic Research 8:73–82
     [Google Scholar]
  34. Wall J. D., Harriman P. D. 1974; Phage Pi mutants with altered transducing abilities for Escherichia coli. Virology 59:532–544
     [Google Scholar]
  35. Watanabe T., Ogata Y., Chan R. K., Botstein D. 1972; Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. I. Transduction of R factor 222 by phage P22. Virology 50:874–882
     [Google Scholar]
  36. Weigle J. 1957; Transduction by coliphage λ of the galactose marker. Virology 4:14–25
     [Google Scholar]
  37. Weigle J., Meselson M., Paigen K. 1959; Density alterations associated with transducing ability in the bacteriophage lambda. Journal of Molecular Biology 1:379–386
     [Google Scholar]
  38. Williams Smith H. 1972; Ampicillin resistance in Escherichia coli by phage infection. Nature; London: 238:205–206
     [Google Scholar]
  39. Yamamoto K. R., Alberts B. M. 1970; Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology 40:734–744
     [Google Scholar]
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Specialized Transduction of Kanamycin Resistance in a Providence Strain
Microbiology 88, 307 (1975); https://doi.org/10.1099/00221287-88-2-307
/content/journal/micro/10.1099/00221287-88-2-307
/content/journal/micro/10.1099/00221287-88-2-307
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