RT Journal Article SR Electronic(1) A1 Delaney, S. F. A1 Carr, N. G.YR 1975 T1 Temporal Genetic Mapping in the Blue-green Alga Anacystis nidulans Using Ethyl Methanesulphonate JF Microbiology, VO 88 IS 2 SP 259 OP 268 DO https://doi.org/10.1099/00221287-88-2-259 PB Microbiology Society, SN 1465-2080, AB SUMMARY: Cultures of the blue-green alga Anacystis nidulans were synchronized with respect to DNA synthesis as well as cell division. Application of ethyl methane-sulphonate at different stages of replication resulted in a peak of mutation frequency for different genetic markers; this peak can be accounted for in terms of the involvement of repair processes. A temporal map of 19 markers has been constructed by this method. Comparison of gene position obtained by temporal mapping indicates that either bidirectional replication or unidirectional replication from more than one origin occurs., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-88-2-259