Deoxyribonucleic Acid Metabolism and Nuclear Division during Spore Germination in Free

Abstract

SUMMARY: Ungerminated microconidia of have a mean cell DNA content of 0·134 × 10 g/cell with a guanine-plus-cytosine composition (% GC) of 50·75%. During germination, the first dry weight increase of the spore population was detected after 3 h incubation and the first germ tube appeared after 4 h. The total DNA of the culture sharply increased after 5 h, followed by a pause at 6 h. At this time the DNA content per nucleus was maximal and the first nuclear divisions were detected. Pauses in the rise of total DNA of the culture and in the [C]adenine incorporation pattern suggest that there is partial synchrony in DNA synthesis at the beginning of incubation. This is also supported by the fact that until 8 h, only hyphae with 1, 2 and 4 nuclei were observed. [C]adenine incorporation into DNA averaged 2·68% of the total taken up in 10 h incubation.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-88-2-245
1975-06-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/88/2/mic-88-2-245.html?itemId=/content/journal/micro/10.1099/00221287-88-2-245&mimeType=html&fmt=ahah

References

  1. Aist J. R., Wilson C. L. 1965; Observations on nuclear division in vegetative hyphaeof Ceratocystis fagacearum. Arkansas Academy of Sciences Proceedings 19:32–36
    [Google Scholar]
  2. Bainbridge B. W. 1971; Macromolecular composition and nuclear division during spore germination in Aspergillus nidulans. Journal of General Microbiology 66:319–325
    [Google Scholar]
  3. Bray G. A. 1960; A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillator counter. Analytical Chemistry 1:279–285
    [Google Scholar]
  4. Cochrane J. C., Rado T. A., Cochrane V. W. 1971; Synthesis of macro-molecules and polyribosome formation in early stages of spore germination in Fusarium solani. Journal of General Microbiology 65:45–55
    [Google Scholar]
  5. De Ley J. 1967; The quick approximation of DNA base composition from absorbancy ratios. Antonie van Leeuwenhoek 33:203–208
    [Google Scholar]
  6. Erwin D. C. 1973; Systemic fungicides: disease control, translocation and mode of action. Annual Review of Phytopathology 11:389–422
    [Google Scholar]
  7. Giles K. W., Myers A. 1965; An improved diphenylamine method for the estimation of DNA. Nature; London: 20693
    [Google Scholar]
  8. Gottlieb D., Van Etten J. 1964; Biochemical changes during the growth of fungi. I. Nitrogen compounds and carbohydrate changes in Penicillium atrovenetum. Journal of Bacteriology 88:114–121
    [Google Scholar]
  9. Gottlieb D., Van Etten J. 1966; Changes in fungi with age. I. Chemical composition of R. solani and R. bataticola. Journal of Bacteriology 91:161–168
    [Google Scholar]
  10. Gottlieb D., Tripathi R. K. 1968; The physiology of swelling phase of spore germination in Penicillium atrovenetum. Mycologia 60:571–590
    [Google Scholar]
  11. Grivell A. R., Jackson J. F. 1968; Thymidine kinase: evidence for its absence from Neurospora crassa and some other micro-organisms, and the relevance of this to the specific labelling of deoxyribonucleic acid. Journal of General Microbiology 54:307–317
    [Google Scholar]
  12. Hollomon D. W. 1970; Ribonucleic acid synthesis during fungal spore germination. Journal of General Microbiology 62:75–87
    [Google Scholar]
  13. Kessel M., Rosenberger R. F. 1968; Regulation and timing of DNA synthesis in hyphae of Aspergillus nidulans. Journal of Bacteriology 95:2275–2281
    [Google Scholar]
  14. Krieg R. E., Lockhart W. R. 1970; Analysis of the thermal transition curves of DNA from microorganisms. Canadian Journal of Microbiology 16:989–995
    [Google Scholar]
  15. Marmur J. 1961; A procedure for the isolation of DNA from microorganisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  16. Marmur J., Doty P. 1962; Determination of the base composition of DNA from its thermal denaturation temperature. Journal of Molecular Biology 5:109–118
    [Google Scholar]
  17. Maruyama Y., Alexander M. 1962; Distribution of protein and nucleic acids in hyphae and micro- conidia of Fusarium. Archiv für Mikrobiologie 41:401–407
    [Google Scholar]
  18. Munro H. N., Fleck A. 1966; Recent development in the measurement of nucleic acids in biological materials. Analyst 91:78–88
    [Google Scholar]
  19. Sijpesteijn A. K. 1970; Biochemical modes of action of agricultural fungicides. World Review of Pest Control 9:85–93
    [Google Scholar]
  20. Sisler H. D. 1968; Effects of fungicides on protein and nucleic acids synthesis. Annual Review of Phytopathology 7:311–330
    [Google Scholar]
  21. Sparrow A. H., Price H. J., Underbrink A. G. 1972; A survey of DNA content per cell and per chromosome of prokaryotic and eukaryotic organisms: some evolutionary considerations. Brookhaven Symposia in Biology 23:451–494
    [Google Scholar]
  22. Storck R. 1972; Deoxyribonucleic acid of fungi. Brookhaven Symposia in Biology 23:371–393
    [Google Scholar]
  23. Van Der Kerk G. J. 1969; The development of synthetic fungicides: trends and prospects. Netherlands Journal of Plant Pathology 75: S1 5–20
    [Google Scholar]
  24. Watson J. D. 1965 The Molecular Biology of the Gene, First edition. New York: W. A. Benjamin;
    [Google Scholar]
  25. Williamson D. A. 1965; The timing of deoxyribonucleic acid synthesis in the cell cycle of Saccharomyces cerevisiae. Journal of Cell Biology 25:517–528
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-88-2-245
Loading
/content/journal/micro/10.1099/00221287-88-2-245
Loading

Data & Media loading...

Most cited Most Cited RSS feed