1887

Abstract

SUMMARY: Bacteroid preparations from nodules of soybean were able to destroy aerobically substantial quantities of indole-3-acetic acid (IAA). Kinetic studies indicate the involvement of two processes. The supernatant derived during preparation of bacteroids had an active IAA oxidation during the first 30 min. Successive washings of bacteroids still retained some activity although it was reduced. A high level of IAA destruction was always observed with washed bacteriods and the reaction proceeded linearly for at least 4 h. Glucose gave a slight stimulation but succinate, pyruvate and fumarate at low concentrations (approx. 25 m) were strong inhibitors. No IAA catabolism occurred with washed bacteroids at a O of 30 mmHg or less, but it increasingly occurred with further increase in oxygen partial pressure up to 150 mmHg. No difference was observed between bacteroids with or without active nitrogenase.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-88-2-223
1975-06-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/88/2/mic-88-2-223.html?itemId=/content/journal/micro/10.1099/00221287-88-2-223&mimeType=html&fmt=ahah

References

  1. Bergersen F. J., Turner G. L. 1967; Nitrogen fixation by the bacteroid fraction of breis of soybean root nodules. Biochimica et biophysica acta 141:507–515
    [Google Scholar]
  2. Bergersen F. J., Turner G. L. 1970; Gel filtration of nitrogenase from soybean root nodule bacteroids. Biochimica et biophysica acta 214:28–36
    [Google Scholar]
  3. Bergersen F. J., Turner G. L. 1973; Kinetic studies of nitrogenase from soybean root nodule bacteroids. Biochemical Journal 131:61–75
    [Google Scholar]
  4. Dullaart J. 1967; Quantitative estimation of indole acetic acid and indole carboxylic acid in root nodules and roots of Lupinus luteus l. Acta botanica neerlandica 16:222–230
    [Google Scholar]
  5. Dullaart J. 1970; The bioproduction of indole-3-acetic acid and related compounds in root nodules and roots of Lupinus luteus l. and by its rhizobial symbiont. Acta botanica neerlandica 19:573–618
    [Google Scholar]
  6. Hardy R. W. F., Holsten R. D., Jackson E. K., Burns R. C. 1968; The acetylene-ethylene assay for N-fixation: laboratory and field evaluation. Plant Physiology 43:1185–1207
    [Google Scholar]
  7. Heller R. 1953; Recherches sur la nutrition minérale des tissus végétaux cultivés in vitro. Annales de Sciences naturelles, de Botanique et de Biologie Végétale1–223
    [Google Scholar]
  8. Mennes A. M. 1972 Indole-3-acetic acid oxidase activity in root nodules and roots of Lupinus luteus l. Purification and kinetics of the enzyme Thesis University of Leiden:
    [Google Scholar]
  9. Pate J. S. 1958; Studies of the growth substances of legume nodules using paper chromatography. Australian Journal of Biological Sciences 11:516–528
    [Google Scholar]
  10. Pilet P. E., Collet G. 1962 Méthode d’Analyse du Catabolisme auxinique Lausanne: Zwahlen;
    [Google Scholar]
  11. Rigaud J. 1969; Croissance, teneur en auxine et catabolisme auxinique chez Rhizobium. Archiv für Mikrobiologie 66:29–33
    [Google Scholar]
  12. Rigaud J., Bergersen F. J., Turner G. L., Daniel R. M. 1973; Nitrate dependent anaerobic acetylene-reduction and nitrogen-fixation by soybean bacteroids. Journal of General Microbiology 77:137–144
    [Google Scholar]
  13. Sequeira L. 1973; Hormone metabolism in diseased plants. Annual Review of Plant Physiology 24:353–380
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-88-2-223
Loading
/content/journal/micro/10.1099/00221287-88-2-223
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error