1887

Abstract

SUMMARY: A soluble extract from able to synthesize the nucleotide precursor of its peptidoglycan was prepared. This extract contained all the enzymes necessary for the synthesis of the peptide side-chain. The specificity of these enzymes was determined and compared with the specificity of similar enzymes extracted from the closely related In both organisms, addition of the third amino acid of the peptide side-chain was specific for the amino acid and nucleotide dipeptide involved in peptidoglycan synthesis in the parent organism. -Diaminobutyric acid, which is found as the acetyl derivative in the precursor nucleotide and in the completed peptidoglycan of was added as the free amino acid and not as the acetylated compound.

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/content/journal/micro/10.1099/00221287-88-1-159
1975-05-01
2021-07-26
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References

  1. Good C. M., Pattee P. A. 1970; Temperature-sensitive osmotically fragile mutants of Staphylococcus aureus. Journal of Bacteriology 104:1401–1403
    [Google Scholar]
  2. Good C. M., Tipper D. J. 1972; Conditional mutants of Staphylococcus aureus defective in cell wall precursor synthesis. Journal of Bacteriology 111:231–241
    [Google Scholar]
  3. Greenstein J. P., Winitz M. 1961 Chemistry of the Amino Acids pp. 2117–2118 New York and London: John Wiley;
    [Google Scholar]
  4. Hammes W. P., Schleifer K. H., Kandler O. 1973; Mode of action of glycine on the biosynthesis of peptidoglycan. Journal of Bacteriology 116:1029–1053
    [Google Scholar]
  5. Hishinuma F., Izaki K., Takahashi H. 1970; Inhibition of incorporation of l-alanine into uridine-diphospho-N-acetylmuramic acid by glycine. Agricultural and Biological Chemistry 34:655–657
    [Google Scholar]
  6. Hishinuma F., Izaki K., Takahashi H. 1971; Inhibition of l-alanine adding enzyme by glycine. Agricultural and Biological Chemistry 35:2050–2058
    [Google Scholar]
  7. Ito E., Nathenson S. G., Dietzler D. N., Anderson J. S., Strominger J. L. 1966; Formation of UDP-acetylmuramyl peptides. Methods in Enzymology 8 pp. 324–337 Neufeld E. F., Ginsburg V. Edited by New York and London: Academic Press;
    [Google Scholar]
  8. Ito E., Strominger J. L. 1962a; Enzymatic synthesis of the peptide in bacterial uridine nucleotides.Enzymatic addition of l-alanine, d-glutamic acid and l-lysine. Journal of Biological Chemistry 237:2689–2695
    [Google Scholar]
  9. Ito E., Strominger J. L. 1962b; Enzymatic synthesis of the peptide in bacterial uridine nucleotides. II.Enzymatic synthesis and addition of d-alanyl-d-alanine. Journal of Biological Chemistry 237:2696–2703
    [Google Scholar]
  10. Ito E., Strominger J. L. 1964; Enzymatic synthesis of the peptide in bacterial uridine nucleotides.III.Purification and properties of l-lysine-adding enzyme. Journal of Biological Chemistry 239:210–214
    [Google Scholar]
  11. Ito E., Strominger J. L. 1973; Enzymatic synthesis of the peptide in bacterial uridine nucleotides. VII. Comparative biochemistry. Journal of Biological Chemistry 248:3131–3136
    [Google Scholar]
  12. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  13. Mizuno Y., Yaegashi M., Ito E. 1973; Purification and properties of uridine diphosphate N-acetylmuramate: l-alanine ligase. Journal of Biochemistry 74:525–538
    [Google Scholar]
  14. Nathenson S. G., Strominger J. L., Ito E. 1964; Enzymatic synthesis of the peptide in bacterial uridine nucleotides. IV. Purification and properties of d-glutamic acid-adding enzyme. Journal of Biological Chemistry 239:1773–1776
    [Google Scholar]
  15. Neuhaus F. C., Carpenter C. V., Miller J. L., Lee N. M., Gragg M., Stickgold R. A. 1969; Enzymatic synthesis of d-alanyl>-d-alanine. Control of d-alanine: d-alanine ligase (ADP). Biochemistry 8:5119–5124
    [Google Scholar]
  16. Nieto M., Perkins H. R., Leyh-Bouille M., Frère J.-M., Ghuysen J.-M. 1973; Peptide inhibitors of Streptomyces dd-carboxypeptidases. Biochemical Journal 131:163–171
    [Google Scholar]
  17. Perkins H. R. 1965; Homoserine in the cell walls of plant-pathogenic corynebacteria. Biochemical Journal 97:3C–5C
    [Google Scholar]
  18. Perkins H. R. 1969; Specificity of combination between mucopeptide precursors and vancomycin or ristocetin. Biochemical Journal 111:195–205
    [Google Scholar]
  19. Perkins H. R. 1971; Homoserine and diaminobutyric acid in the mucopeptide-precursor-nucleotides and cell walls of some plant-pathogenic corynebacteria. Biochemical Journal 121:417–423
    [Google Scholar]
  20. Reissig J. L., Strominger J. L., Leloir L. F. 1955; A modified colorimetric method for the estimation of N-acetylamino sugars. Journal of Biological Chemistry 217:959–966
    [Google Scholar]
  21. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriological Reviews 36:407–477
    [Google Scholar]
  22. Strominger J. L., Threnn R. H. 1959; Accumulation of a uridine nucleotide in Staphylococcus aureus as the consequence of lysine deprivation. Biochimica et biophysica acta 36:83–92
    [Google Scholar]
  23. Tipper D. J., Pratt I. 1970; Cell wall polymers of Bacillus sphaericus 9602. II. Synthesis of the first enzyme unique to cortex synthesis during sporulation. Journal of Bacteriology 103:305–317
    [Google Scholar]
  24. Ward J. B. 1974; The synthesis of peptidoglycan in an autolysin-deficient mutant of Bacillus licheniformis N.C.T.C. 6346 and the effect of β-lactam antibiotics, bacitracin and vancomycin. Biochemical Journal 141:227–241
    [Google Scholar]
  25. Watanabe Y., Shimura K. 1956; Biosynthesis of threonine from homoserine. V. Nature of an intermediary product. Journal of Biochemistry 43:283–294
    [Google Scholar]
  26. Wishnow R. M., Strominger J. L., Birge C. H., Threnn R. H. 1965; Biochemical effects of novobiocin on Staphylococcus aureus. Journal of Bacteriology 89:1117–1123
    [Google Scholar]
  27. Wright H. D. 1933; The importance of adequate reduction of peptone in the preparation of media for the pneumococcus and other organisms. Journal of Pathology and Bacteriology 37:257–281
    [Google Scholar]
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