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Volume 88, Issue 1

The Genetics of Tetracycline Resistance in Free

Abstract

SUMMARY: Eighty-one strains of that appeared to be tetracycline resistant on the basis of a preliminary disc-diffusion test were examined for resistance to tetracycline and to the semi-synthetic tetracycline, minocycline. Minimum inhibitory concentration (m.i.c.) values for both drugs were determined after induction of the strains by growth for 2 h in sub-inhibitory concentrations of tetracycline. Forty-seven strains (58%) had m.i.c. values for minocycline of 12·5 g/ml or greater, and were considered to be minocycline resistant. An additional ten strains had m.i.c. values for minocycline of 3·125 to 6·25 g/ml and were classified as low-level resistant strains. It appears, therefore, that a fairly high proportion of tetracycline-resistant strains isolated at the present time are resistant to concentrations of minocycline unattainable with the recommended dosage for this antibiotic (Frisk & Tunevall, 1969).

Transductional analysis of the genetic determinants for tetracycline resistance revealed the existence of two types of resistance to high concentrations of tetracycline. Strains in the first category (A) were inducibly resistant to tetracycline but sensitive to minocycline; in these strains the resistance determinant was plasmid-borne. Strains in the second category (B) were resistant to both tetracycline and minocycline and had low induction ratios for tetracycline resistance; the genetic determinant for resistance in these strains was chromosomal. In addition, certain strains in category A were found to carry a chromosomal gene controlling low-level resistance to tetracycline and minocycline. This low-level resistance to tetracycline was masked in the presence of the tetracycline plasmid but could be demonstrated after loss of the plasmid. The results suggest that more than one mechanism of resistance to tetracyclines may exist in staphylococci.

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© Society for General Microbiology, 1975
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1975-05-01
2024-12-13
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References

  1. Arber W. 1960; Transduction of chromosomal genes and episomes in Escherichia coli. Virology 11:273–288
     [Google Scholar]
  2. Asheshov E. H. 1966a; Loss of antibiotic resistance in Staphylococcus aureus resulting from growth at high temperature. Journal of General Microbiology 42:403–410
     [Google Scholar]
  3. Asheshov E. H. 1966b; Chromosomal location of the genetic elements controlling penicillinase production in a strain of Staphylococcus aureus. Nature; London: 210804–806
     [Google Scholar]
  4. Asheshov E. H. 1969; The genetics of penicillinase production in Staphylococcus aureus strain PS80. Journal of General Microbiology 59:289–301
     [Google Scholar]
  5. Chopra I., Bennett P., Lacey R. W. 1973; A variety of staphylococcal plasmids present in multiple copies. Journal of General Microbiology 79:343–345
     [Google Scholar]
  6. Fedorko J., Katz S., Allnoch H. 1968; In vitro activity of minocycline, a new tetracycline. American Journal of Medical Science 255:252–258
     [Google Scholar]
  7. Franklin T. J., Godfrey A. 1965; Resistance of Escherichia coli to tetracyclines. Biochemical Journal 94:54–60
     [Google Scholar]
  8. Frisk A. R., Tunevall G. 1969; Clinical evaluation of minocycline. In Antimicrobial Agents and Chemotherapy-1968 pp. 335–339
     [Google Scholar]
  9. Inoue M., Hashimoto M., Mitsuhashi S. 1970; Mechanism of tetracycline resistance in Staphylococcus aureus. I. Inducible resistance to tetracycline. Journal of Antibiotics 23:68–74
     [Google Scholar]
  10. Izaki K., Arima K. 1963; Disappearance of oxytetracycline accumulation in the cells of multiple drug-resistant Escherichia coli. Nature; London: 200384–385
     [Google Scholar]
  11. Kayser F. H., Wüst J., Corrodi P. 1972; Transduction and elimination of resistance determinants in methicillin-resistant Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 2:217–223
     [Google Scholar]
  12. Kuck N. A., Forbes M. 1973; Uptake of minocycline and tetracycline by tetracycline-susceptible and -resistant bacteria. Antimicrobial Agents and Chemotherapy 3:662–664
     [Google Scholar]
  13. Lacey R. W. 1973; Genetic basis, epidemiology and future significance of antibiotic resistance in Staphylococcus aureus. A review. Journal of Clinical Pathology 26:899–913
     [Google Scholar]
  14. Leigh D. A., Simmons K. 1974; Effect of minocycline on tetracycline-resistant Staphylococcus aureus. Lancet i:1006
     [Google Scholar]
  15. May J. W., Houghton R. H., Perret C. J. 1964; The effect of growth at elevated temperatures on some heritable properties of Staphylococcus aureus. Journal of General Microbiology 37:157–169
     [Google Scholar]
  16. Novick R. P., Bouanchaud D. 1971; Extrachromosomal nature of drug resistance in Staphylococcus aureus. Annals of the New York Academy of Sciences 182:279–294
     [Google Scholar]
  17. Poston S. M. 1966; Cellular location of the genes controlling penicillinase production and resistance to streptomycin and tetracycline in a strain of Staphylococcus aureus. Nature; London: 210802–804
     [Google Scholar]
  18. Sasaki K., Suzue G., Ishihara K., Odani S., Tanaka S. 1970; Mechanism of resistance of Staphylococcus aureus to tetracyclines. Journal of General and Applied Microbiology 16:145–159
     [Google Scholar]
  19. Schaefler S., Francois W. 1973; Minocycline-resistant mutants in Staphylococcus aureus. In Abstract II, International Congress of Bacteriology Jerusalem: p. 30:
     [Google Scholar]
  20. Sompolinsky D., Zaidenzaig Y., Ziegler-Schlomowitz R., Abramova N. 1970; Mechanism of tetracycline resistance in Staphylococcus aureus. Journal of General Microbiology 62:351–367
     [Google Scholar]
  21. Weaver J. R., Pattee P. A. 1964; Inducible resistance to erythromycin in Staphylococcus aureus. Journal of Bacteriology 88:574–580
     [Google Scholar]
  22. Wyman L., Goering R. V., Novick R. P. 1974; Genetic control of chromosomal and plasmid recombination in Staphylococcus aureus. Genetics 76:681–702
     [Google Scholar]
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The Genetics of Tetracycline Resistance in Staphylococcus aureus
Microbiology 88, 132 (1975); https://doi.org/10.1099/00221287-88-1-132
/content/journal/micro/10.1099/00221287-88-1-132
/content/journal/micro/10.1099/00221287-88-1-132
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