1887

Microbiology

Volume 88, Issue 1

Studies on the ‘Osmophilic’ Yeast and an Obligate Osmophilic Mutant Free

Abstract

SUMMARY: An obligate osmophilic mutant (strain B1/4) of has been isolated that fails to grow at osmotic pressures corresponding to 20% (w/v) sucrose or less. In 30% sucrose the yeast is filamentous and grows slowly. In 40% sucrose it is mainly filamentous and has over twice the normal diameter. In 60% sucrose it grows in the yeast form with a growth rate twice that of the culture in 40% sucrose. This mutant is lysed by a sudden drop in the osmotic pressure of the environment. Cell envelopes of the parent strain contained glucose and mannose in the ratio 1.2:1 and contained 3·8% (w/v) hexosamine, whereas the envelopes of the mutant contained 0·8% hexosamine. Cell envelopes of the mutant grown in 40% sucrose contained glucose and mannose in the ratio 1·9:1, whereas for envelopes of the yeast grown in 60% sucrose the ratio was 1·2:1. Neutral lipids from whole cells and those from the envelopes of the mutant strain generally contained more unsaturated fatty acids than the corresponding fractions from the parent strain.

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© Society for General Microbiology, 1975
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1975-05-01
2021-10-23
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Studies on the ‘Osmophilic’ Yeast Saccharomyces rouxii and an Obligate Osmophilic Mutant
Microbiology 88, 101 (1975); https://doi.org/10.1099/00221287-88-1-101
/content/journal/micro/10.1099/00221287-88-1-101
/content/journal/micro/10.1099/00221287-88-1-101
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