1887

Abstract

SUMMARY: Spores of contain a toxin active against lepidopterous larvae. This toxin can be solubilized extraction with reagents which dissolve the protein crystal of It is inactivated crystal-specific antiserum. Spores of contain a similar toxin although the specific activity is much lower than the spores of The toxin contains a single major polypeptide component. Toxic activity can be solubilized from spores of both species incubation with gut juices from

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/content/journal/micro/10.1099/00221287-87-2-359
1975-04-01
2022-01-25
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References

  1. Cooksey K. E. 1968; Purification of a protein from Bacillus thuringiensis toxic to larvae of Lepidoptera. Biochemical Journal 106:445–454
    [Google Scholar]
  2. Crowle A. J. 1961 Immunodiffusion New York: Academic Press;
    [Google Scholar]
  3. Delafield F. P., Somerville H. J., Rittenberg S. C. 1968; Immunological homology between crystal and spore protein of Bacillus thuringiensis. Journal of Bacteriology 96:713–720
    [Google Scholar]
  4. Ellis D. A. 1961; A new universal buffer. Nature; London: 1911099
    [Google Scholar]
  5. Herbert B. N., Gould H. J. 1973; Biosynthesis of the crystal protein of Bacillus thuringiensis var. tolworthi. I. Kinetics of formation of the polypeptide components of the crystal protein in vivo. European Journal of Biochemistry 37:441–448
    [Google Scholar]
  6. Herbert B. N., Gould H. J., Chain E. B. 1971; Crystal protein of Bacillus thuringiensis var. tolworthi. Subunit structure and toxicity to Pieris brassicae. European Journal of Biochemistry 24:366–375
    [Google Scholar]
  7. Lecadet M. M., Chevrier G., Dedonder R. 1972; Analysis of a protein fraction in the spore coats of Bacillus thuringiensis. European Journal of Biochemistry 25:349–358
    [Google Scholar]
  8. Lecadet M. M., Dedonder R. 1971; Biogenesis of the crystalline inclusion of Bacillus thuringiensis during sporulation. European Journal of Biochemistry 23:282–294
    [Google Scholar]
  9. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  10. Raybin D. M., Bertsch L. L., Kornberg A. 1972; A phospholipase in Bacillus megaterium unique to spores and sporangia. Biochemistry 11:1754–1760
    [Google Scholar]
  11. Ribier J. 1971; L’inclusion parasporale du Bacillus thuringiensis var. Berliner 1715: moment et site de son initiation, rapports avec l’ADN sporangial. Comptes rendus hebdomadaire des seances de l’Academie des sciences 273:1444–1447
    [Google Scholar]
  12. Ribier J., Lecadet M. M. 1973; Étude ultrastructurale et kinetique de la sporulation de Bacillus thuringiensis, 1715. Remarques sur la formation de l’inclusion parasporale. Annales de Microbiologie (Institut Pasteur) 124A:311–344
    [Google Scholar]
  13. Scherrer P., Pillinger R. N., Somerville H. J. 1974; Toxicity to Lepidoptera in the exosporium membrane of spores of Bacillus thuringiensis. Proceedings of the Society for General Microbiology 145
    [Google Scholar]
  14. Shapiro A. L., Viñuela E., Maizel J. V., Jun J. V. 1967; Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochemical and Biophysical Research Communications 28:815–820
    [Google Scholar]
  15. Short J., Walker P., Thomson R. O., Somerville H. J. 1974; The fine structure of Bacillus finitimus and Bacillus thuringiensis spores with special reference to the location of crystal antigen. Journal of General Microbiology 84:261–276
    [Google Scholar]
  16. Somerville H. J. 1971; Formation of the parasporal inclusion of Bacillus thuringiensis. European Journal of Biochemistry 18:226–237
    [Google Scholar]
  17. Somerville H. J., Delafield F. P., Rittenberg S. C. 1968; Biochemical homology between crystal and spore protein of Bacillus thuringiensis. Journal of Bacteriology 96:721–726
    [Google Scholar]
  18. Somerville H. J., Delafield F. P., Rittenberg S. C. 1970; Urea-mercaptoethanol-soluble protein from spores of Bacillus thuringiensis and other species. Journal of Bacteriology 101:551–560
    [Google Scholar]
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