RT Journal Article SR Electronic(1) A1 Cornelis, G. A1 Abraham, E. P.YR 1975 T1 β-Lactamases from Yersinia enterocolitica JF Microbiology, VO 87 IS 2 SP 273 OP 284 DO https://doi.org/10.1099/00221287-87-2-273 PB Microbiology Society, SN 1465-2080, AB SUMMARY: Two β-lactamases, A and B, have been shown to be present in a strain of Yersinia enterocolitica (W222). β-Lactamase A hydrolyses a variety of penicillins and cephalosporins. This enzyme is sensitive to thiol reagents, is only partially inhibited 0·1 mm-cloxacillin and has a molecular weight of approximately 20000. β-Lactamase B shows strong cephalosporinase activity but does not hydrolyse some of the penicillins. It is more resistant than β-lactamase A to thiol reagents, is completely inhibited 0·1 mm-cloxacillin and has a molecular weight of about 34000. With cephaloridine as a substrate, which is readily hydrolysed both enzymes, about 85% of the total activity of a cell extract is due to β-lactamase A and 15% to B. Addition of 6-aminopenicillanic acid to the culture during growth results in a 2- to 4-fold selective increase in the amount of β-lactamase B. Two β-lactamases similar to enzymes A and B have been found in five other strains of Y. enterocolitica. In contrast, only one β-lactamase, similar to enzyme B, has been detected in a different strain of Y. enterocolitica (H66), which is abnormal in that it is sensitive to ampicillin. Addition of 6-aminopenicillanic acid to cultures of this strain results in an 8- to 10-fold increase in β-lactamase production., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-87-2-273