1887

Abstract

SUMMARY: An agar-degrading bacterium, having a guanine-cytosine content of 50·5 mol% has been isolated from sewage. This Gram-negative rod grew well in a simple salts medium containing various carbohydrates.

Growing bacteria dissolved gels and suspensions of agar and agarose rapidly, but did not attack cross-linked agars. Agarase was cell-bound in exponentially growing cultures but was released into the medium at stationary phase. Both cell extracts and culture filtrates released reducing sugars from agar solutions and prevented them from gelling. Gels were not dissolved by enzyme solutions, but the turbidity and iodine-binding properties of the agar were decreased.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-87-1-150
1975-03-01
2021-07-31
Loading full text...

Full text loading...

/deliver/fulltext/micro/87/1/mic-87-1-150.html?itemId=/content/journal/micro/10.1099/00221287-87-1-150&mimeType=html&fmt=ahah

References

  1. Araki C., Arai K. 1954; Studies on agar-digesting bacteria. The isolation of agar-digesting bacteria and their enzymatic activities. Memoirs of the Faculty of Industrial Arts, Kyoto Technical University, Science and Technology 3B:7–23
    [Google Scholar]
  2. Araki C., Arai K. 1956; Studies on the chemical constitution of agar-agar. XVIII. Isolation of a new crystalline disaccharide by enzymatic hydrolysis of agar-agar. Bulletin of the Chemical Society of Japan 29(3):339–345
    [Google Scholar]
  3. AxÉn R., Porath J., Ernback S. 1967; Chemical coupling of proteins to agarose. Nature; London: 2151491–1492
    [Google Scholar]
  4. Baumann L., Baumann P., Mandel M., Allen R. D. 1972; Taxonomy of aerobic marine eubacteria. Journal of Bacteriology 110:402–429
    [Google Scholar]
  5. Duckworth M., Yaphe W. 1971a; The structure of agar. I. Fractionation of a complex mixture of polysaccharides. Carbohydrate Research 16:189–197
    [Google Scholar]
  6. Duckworth M., Yaphe W. 1971b; The structure of agar. II. The use of a bacterial agarase to elucidate structural features of the charged polysaccharides in agar. Carbohydrate Research 16:435–445
    [Google Scholar]
  7. V.Hofsten B., Edberg N. 1972; Estimating the rate of degradation of cellulose fibers in water. Oikos 23:29–34
    [Google Scholar]
  8. Izumi K. 1972; Chemical heterogeneity of the agar from Gracilaria verrucosa. Journal of Biochemistry 72:135–140
    [Google Scholar]
  9. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  10. Miller J., Blum R., Glennon W. E., Barton A. L. 1960; Measurement of carboxylmethyl cellulase activity. Analytical Biochemistry 1:127–132
    [Google Scholar]
  11. Ng Ying Kin N. M. K., Yaphe W. 1972; Properties of agar. Parameters affecting gel formation and the agarose-iodine reaction. Carbohydrate Research 25:379–385
    [Google Scholar]
  12. Porath J., Janson J.-C., LÅÅs T. 1971; Agar derivatives for chromatography, electrophoresis and gel-bound enzymes. I. Desulphated and reduced cross-linked agar and agarose in spherical bead form. Journal of Chromatography 60:167–177
    [Google Scholar]
  13. Rees D. A. 1972; Shapely polysaccharides. Biochemical Journal 126:257–272
    [Google Scholar]
  14. Sampietro A. R., Vattuone De Sampietro M. A. 1971; Characterization of the agarolytic system of Agarbacterium pastinator. Biochimica et biophysica acta 244:65–76
    [Google Scholar]
  15. Shewan J. M., Hobbs G., Hodgkiss W. 1960; A determinative scheme for the identification of certain genera of Gram-negative bacteria, with special reference to the Pseudomonadaceae. Journal of Applied Bacteriology 23:379–390
    [Google Scholar]
  16. Svennerholm L. 1956; The quantitative estimation of cerebrosides in nervous tissues. Journal of Neuro-chemistry 1:42–53
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-87-1-150
Loading
/content/journal/micro/10.1099/00221287-87-1-150
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error