SUMMARY: Physical and nutritional factors required for growth of isolates from ovine foot-rot lesions were examined. Simplified anaerobic culture techniques were devised utilizing a fully soluble, autoclavable, liquid medium (TAS) which contained proteose-peptone, yeast and meat extracts and certain other essential compounds required to promote prompt and serially transferrable growth of cultures from small inocula. The latter included Trypticase, arginine, a reducing agent (most suitably thioglycollic acid) and CO; serine and Mg markedly increased growth yields. Trypticase could not be replaced by a commercial preparation of acid-hydrolysed casein; other forms of hydrolysed proteins gave delayed and inconsistent growth. Maximum growth of cultures required concentrations of 0.02 to 0.05 M-arginine, which could not be replaced by glutamic acid, citrulline or ornithine. Exogenous carbohydrate compounds were not required.

The temperature range for optimum growth of cultures was 37 to 39 °C, and anaerobic culture conditions were essential for growth and the production of organisms of normal morphology. Solidified TAS media for the isolation and maintenance of cultures were also devised.


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