1887

Abstract

SUMMARY: Several mutants obtained from smooth strains by selection for resistance to Felix O (FO) phage [whose receptor site includes the -acetylglucosamine branch of the lipopolysaccharide (LPS) core] were smooth in cultural properties, antigenic character and phage sensitivity pattern (except for their FO resistance). However, the affected genes of several such ‘FOR’ (FO-resistant) mutants were shown by transduction to map in the short segment, which includes nearly all known genes responsible for synthesis of LPS core. All of seven FOR mutants differed from their parents, and resembled mutants with defects in the deeper part of the LPS core, by increased sensitivity to various antibiotics. One FOR mutant was non-virulent (LD > 10, compared with < 100 for its parent); 7 derivatives given this FOR gene by co-transduction with were likewise non-virulent. It is inferred that FOR mutations affect the assembly of the inner part of the LPS core, perhaps causing incomplete blocks in glycosyl transferase reactions.

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1975-03-01
2024-12-05
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References

  1. Gemski P., Stocker B. A. D. 1967; Transduction by phage P22 in nonsmooth mutants of Salmonella typhimurium. Journal of Bacteriology 93:1588–1597
    [Google Scholar]
  2. Hellerqvist C. G., Lindberg A. A. 1971; Structural studies of the common-core polysaccharide from Salmonella typhimurium. Carbohydrate Research 16:39–48
    [Google Scholar]
  3. Kallings L. O. 1967; Sensitivity of various Salmonella strains to Felix O’1 phage. Acta pathologica et microbiologica scandinavicci 70:446–460
    [Google Scholar]
  4. Krishnapillai V. 1971; IJDPgalactose-epimerase deficiency in Salmonella typhimurium and its correction by plasmid-borne galactose genes of E. coli KI2: effects on mouse virulence. Infection and Immunity 4:177–188
    [Google Scholar]
  5. Krishnapillai V., Macphee D. G., Stocker B. A. D. 1971; Properties of a Salmonella typhimurium mutant with an incomplete deficiency of UDP galactose-4-epimerase. Journal of Bacteriology 107:155–161
    [Google Scholar]
  6. Kuo T. -T., Stocker B. A. D. 1972; Mapping of rfa genes in Salmonella typhimurium by ES18 and P22 transduction and by conjugation. Journal of Bacteriology 112:48–63
    [Google Scholar]
  7. Lehmann V., Hämmerling G., Nurminen M., Minner I., Ruschmann E., Lüderitz O., Kuo T. -T., Stocker B. A. D. 1973; A new class of heptose-defective mutant of Salmonella typhimurium. European Journal of Biochemistry 32:268–275
    [Google Scholar]
  8. Lindberg A. A. 1973; Bacteriophage receptors. Annual Review of Microbiology 27:205–241
    [Google Scholar]
  9. Lindberg A. A., Hellerqvist C. G. 1971; Bacteriophage attachment sites, serological specificity and chemical composition of the lipopolysaccharides of semirough and rough mutants of Salmonella typhimurium. Journal of Bacteriology 105:57–64
    [Google Scholar]
  10. Lindberg A. A., Holme T. 1969; Influence of O side chains on the attachment of the Felix O-i bacteriophage to Salmonella bacteria. Journal of Bacteriology 99:513–519
    [Google Scholar]
  11. Lindberg A. A., Svensson S. 1975; Salmonella typhimurium mutations conferring resistance to Felix O phage without loss of smooth character: phage attachment and immunochemical and structural analyses of lipopolysaccharides. Journal of General Microbiology 87:11–19
    [Google Scholar]
  12. Macphee D. G., Stocker B. A. D. 1969; Suppression of amber and ochre mutants in Salmonella typhimurium by a mutant F′-1 -gal factor carrying an ochre suppressor gene. Journal of Bacteriology 100:240–246
    [Google Scholar]
  13. Morgenroth A., Duguid J. P. 1968; Demonstration of different mutational sites controlling rhamnose fermentation in FIRN and non-FIRN rha strains of Salmonella typhimurium. Genetical Research 11:151–169
    [Google Scholar]
  14. Mühlradt P. F. 1971; Biosynthesis of Salmonella lipopolysaccharide. Studies on the transfer of glucose, galactose and phosphate to the core in a cell-free system. European Journal of Biochemistry 18:20–27
    [Google Scholar]
  15. Naide Y., Nikaido H., Mäkelä P. H., Wilkinson R. G., Stocker B. A. D. 1965; Semirough strains of Salmonella. Proceedings of the National Academy of Sciences of the United States of America 53:147–153
    [Google Scholar]
  16. Roantree R. J., Kuo T.-T., Macphee D. G., Stocker B. A. D. 1969; Effect of various rough lesions in Salmonella typhimurium upon sensitivity to antibiotics. Bacteriological Proceedings p. 79
    [Google Scholar]
  17. Sanderson K. E., Ross H., Ziegler L., Mäkelä P. H. 1972; F+, Hfr and F′ strains of Salmonella typhimurium and Salmonella abony. Bacteriological Reviews 38:608–637
    [Google Scholar]
  18. Sanderson K. E., Saeed H. 1972; Insertion of the F factor into the cluster of rfa (Rough A) genes of Salmonella typhimurium. Journal of Bacteriology 112:64–73
    [Google Scholar]
  19. Schlecht S., Schmidt G. 1969; Moglichkeiten zur Differenzierung von Salmonella-R-Formen mittels Antibiotica und antibakterieller Farbstoffe. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene Abteilung I 212:505–511
    [Google Scholar]
  20. Steward J. P., Collis L. R., Roantree R. J. 1966; Effects of immunization of guinea pigs lacking bactericidal antibody against Salmonella enteritidis. Journal of Immunology 97:224–230
    [Google Scholar]
  21. Subbiah T. V. 1963 Genetics of virulence in Salmonella typhimurium; mapping of rough and other loci concerned Ph.D. Thesis University of London:
    [Google Scholar]
  22. Wilkinson R. G., Gemski P., Stocker B. A. D. 1972; Non-smooth mutants of Salmonella typhimurium: differentiation by phage sensitivity and genetic mapping. Journal of General Microbiology 70:527–554
    [Google Scholar]
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