1887

Abstract

SUMMARY: liquid surface cultures on semi-defined medium with glucose as carbon source passed through well-defined phases corresponding to trophophase, idiophase (enniatin production), and a degenerative phase. All the glucose was consumed by day 6, at which time the mycelial dry weight had reached only half its maximum. When glucose was replaced by lactose, there was no separation of trophophase and idiophase. Enniatin production, dry weight, and sugar and nitrogen consumption were in approximate balance throughout the growth period (25 days), after which slow degeneration began. The term ‘unitary metabolism’ is proposed for this type of unphased behaviour. Unitary metabolism may approximate more closely to that occurring under natural conditions than does the metabolic phase separation observed when rapidly utilized carbon sources are used in laboratory cultures.

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/content/journal/micro/10.1099/00221287-86-2-327
1975-02-01
2021-05-09
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References

  1. Audhya T. K., Russell D. W. 1973a; Secondary metabolism - biological phenomenon or semantic quibble?. Proceedings of the Canadian Federation of Biological Societies 16:39
    [Google Scholar]
  2. Audhya T. K., Russell D. W. 1973b; Spectrophotometric determination of enniatin A and valinomycin in fungal extracts by ion complexation. Analytical Letters 6:265–274
    [Google Scholar]
  3. Audhya T. K., Russell D. W. 1973c; Production of enniatin A. Canadian Journal of Microbiology 19:1051–1054
    [Google Scholar]
  4. Audhya T. K., Russell D. W. 1974a; Production of enniatins by Fusarium sambucinunv. selection of high-yield conditions from liquid surface cultures. Journal of General Microbiology 82:181–190
    [Google Scholar]
  5. Audhya T. K., Russell D. W. 1974b; Natural enniatin A, a mixture of optical isomers containing both erythro- and threo-N-methyl-l-isoleucine residues. Journal of the Chemical Society Perkin Transactions 1:743–746
    [Google Scholar]
  6. Bertaud W. S., Morice I. M., Russell D. W., Taylor A. 1963; The spore surface in Pithomyces chartarum. Journal of General Microbiology 32:385–395
    [Google Scholar]
  7. Bishop E., Griffiths H., Russell D. W., Ward V., Gartside R. N. 1965; Spore surface depsipeptides in some Pithomyces species. Journal of General Microbiology 38:289–297
    [Google Scholar]
  8. Borrow A., Jefferys E. G., Kessell R. H. J., Lloyd E. C., Lloyd P. B., Nixon E. S. 1961; The metabolism of Gibberella fujikuroi in stirred culture. Canadian Journal of Microbiology 7:227–276
    [Google Scholar]
  9. Bu’Lock J. D. 1961; Intermediary metabolism and antibiotic synthesis. Advances in Applied Microbiology 3:293–342
    [Google Scholar]
  10. Bu’Lock J. D. 1967 Essays in Biosynthesis and Microbial Development pp. 49–58 New York: John Wiley;
    [Google Scholar]
  11. Demain A. L. 1968; Regulatory mechanisms and the industrial production of microbial metabolites. Lloydia 31:395–418
    [Google Scholar]
  12. Done J., Mortimer P. H., Taylor A., Russell D. W. 1961; The production of sporidesmin and spori- desmolides by Pithomyces chartarum. Journal of General Microbiology 26:207–222
    [Google Scholar]
  13. Foster J. W. 1949 Chemical Activities of Fungi pp. 164–169 New York: Academic Press;
    [Google Scholar]
  14. Gallo M., Katz E. 1972; Regulation of secondary metabolite biosynthesis: catabolite repression of phenoxazinone synthase and actinomycin formation by glucose. Journal of Bacteriology 109:659–667
    [Google Scholar]
  15. Gäaumann E., Roth S., Ettlinger L., Plattner P. A., Nager U. 1947; Enniatin, ein neues, gegen Mykobakterien wirksames Antibiotikum. Experientia 3:202–203
    [Google Scholar]
  16. Haavik H. I. 1974; Studies on the formation of bacitracin by Bacillus licheniformis: effect of glucose. Journal of General Microbiology 81:383–390
    [Google Scholar]
  17. Hanes C. S. 1929; An application of the method of Hagedorn and Jensen to the determination of larger quantities of reducing sugars. Biochemical Journal 23:99–106
    [Google Scholar]
  18. Hesseltine C. W. 1972; Solid state fermentations. Biotechnology and Bioengineering 14:517–532
    [Google Scholar]
  19. Johnson M. J. 1952; Recent advances in penicillin fermentation. Bulletin of the World Fiealth Organization 6:99–121
    [Google Scholar]
  20. Malpress F. H., Morrison A. B. 1949; The semi-micro estimation of lactose alone and in the presence of other sugars. Biochemical Journal 45:455–459
    [Google Scholar]
  21. Partridge S. M. 1948; Filter-paper partition chromatography of sugars. Biochemical Journal 42:238–250
    [Google Scholar]
  22. Raunio R. 1966; The effects of pyruvate and related compounds on the induced formation of tryptophanase in Escherichia coli. Acta chemica scandinavica 20:17–23
    [Google Scholar]
  23. Riches P., Rothwell A., Russell D. W. 1967; The spore-surface depsipeptide of Pithomyces sacchari. Journal of General Microbiology 46:77–83
    [Google Scholar]
  24. Russell D. W. 1966; Cyclodepsipeptides. Quarterly Reviews of the Chemical Society 20:559–576
    [Google Scholar]
  25. Saloman L. L., Johnson J. E. 1959; Enzymatic microdetermination of glucose in blood and urine. Analytical Chemistry 31:453–456
    [Google Scholar]
  26. Saltero F. V., Johnson M. J. 1953; The effect of carbohydrate nutrition on penicillin production by Penicillium chrysogenum Q-176. Applied Microbiology 1:52–57
    [Google Scholar]
  27. Taber W. A., Siepmann R. 1965; Measurement of growth in liquid cultures of molds. Applied Microbiology 13:827–829
    [Google Scholar]
  28. Weinberg E. D. 1971; Secondary metabolism: raison d’être. Perspectives in Biology and Medicine 14:565–577
    [Google Scholar]
  29. Woodruff H. B. 1966; Biochemical studies of antimicrobial drugs. Symposia of the Society for General Microbiology 16:22–46
    [Google Scholar]
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