1887

Abstract

Extraction of mycelium or walls of with cold or hot aqueous phenol yielded a lipopolysaccharide consisting of lipid A, phosphate, galactose, arabinose, glucose, glucosamine, and a dideoxy sugar. Extraction with trichloro-acetic acid (TCA) yielded an incomplete molecule lacking lipid A. Part of an O-chain was secreted into the culture medium. Phenol and TCA extracts gave three lines of precipitation with human serum from cases of farmer’s lung disease, and one of these was given by the culture medium polysaccharide. Serologically-reactive sugars were arabinose, galactose and glucose. The lipopolysaccharide fixed on to red cells which agglutinated in the presence of specific antibody and lysed on the addition of complement. The lipopolysaccharide appeared to elicit mainly IgM antibodies in animals, but IgM and IgG antibodies in humans.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-86-2-250
1975-02-01
2021-05-08
Loading full text...

Full text loading...

/deliver/fulltext/micro/86/2/mic-86-2-250.html?itemId=/content/journal/micro/10.1099/00221287-86-2-250&mimeType=html&fmt=ahah

References

  1. Aminoff D. 1961; Methods for the quantitative estimation of n-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochemical Journal 81:384–392
    [Google Scholar]
  2. Costerton J. W., Ingram J. M., Cheng K. -J. 1974; Structure and function of the cell envelope of Gram-negative bacteria. Bacteriological Reviews 38:87–110
    [Google Scholar]
  3. Dische Z. 1953; Qualitative and quantitative colorimetric determination of heptoses. Journal of Biological Chemistry 204:983–997
    [Google Scholar]
  4. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  5. Fensom A. H., Gray A. W. 1969; The chemical composition of the lipopolysaccharide of Pseudomonas aeruginosa. Biochemical Journal 114:185–195
    [Google Scholar]
  6. Frank M. M., May J. E., Kane M. A. 1973; Contributions of the classical and alternate complement pathways to the biological effects of endotoxin. Journal of Infectious Diseases 128:76–181
    [Google Scholar]
  7. Gewurz H., Shin H. S., Mergenhagen S. E. 1968; Interactions of the complement system with endotoxic lipopolysaccharide: consumption of each of the six terminal complement components. Journal of Experimental Medicine 128:1049–1057
    [Google Scholar]
  8. Ghalambor M. A., Levine E. M., Heath E. C. 1966; The biosynthesis of cell wall lipopolysaccharide in Escherichia coli. III. The isolation and characterisation of 3-deoxyoctulosonic acid. Journal of Biological Chemistry 241:3207–3215
    [Google Scholar]
  9. Goldstein I. J., Hollerman C. E., Smith E. E. 1965; Protein-carbohydrate interaction. II. Inhibition studies on the interaction of concanavalin A with polysaccharides. Biochemistry 4:876–883
    [Google Scholar]
  10. Greisman S. E., Hornick R. B. 1973; Mechanisms of endotoxin tolerance with special reference to man. Journal of Infectious Diseases 128:S265–276
    [Google Scholar]
  11. Hollingdale M. R. 1974; Antibody responses in patients with farmer’s lung disease to antigens from Micropolyspora faeni. Journal of Hygiene 72:79–89
    [Google Scholar]
  12. Hollingdale M. R., Murray I. G. 1974; Antibody responses in patients with farmer’s lung disease. In Proceedings of the Fourth International Congress on Aspergillosis and Farmer’s Lung Davos pp. 280–287 Bern, Switzerland: Hans Huber;
    [Google Scholar]
  13. Ikawa M., Koepfli J. B., Mudd S. G., Niemann C. 1953; An agent from E. coli causing hemorrhage and regression of an experimental mouse tumor. III. The component of fatty acids of the phospholipide moiety. Journal of the American Chemical Society 75:1035–1038
    [Google Scholar]
  14. Janda J., Work E. 1971; A colorimetric estimation of lipopolysaccharides. FEBS Letters 16:343–345
    [Google Scholar]
  15. Kabat E. A., Mayer M. M. 1961 ExperimentalImmunochemistry, 2nd edn. p. 260 Springfield, Illinois: Charles C. Thomas;
    [Google Scholar]
  16. Kochman S., Martin J. -C, Bureau G., Dubois De Montreynald J. -M. 1972; Pulmonary reactions to Micropolyspora faeni in sensitized rabbits. Clinical Allergy 2:307–315
    [Google Scholar]
  17. Loewus F. A. 1952; Improvement in anthrone method for determination of carbohydrates. Analytical Chemistry 24:219–224
    [Google Scholar]
  18. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  19. Pepys J., Jenkins P. A. 1965; Precipitin (FLH) test in farmer’s lung. Thorax 20:21–35
    [Google Scholar]
  20. Pepys J. 1969; Hypersensitivity diseases of lungs due to fungi and organic dusts. Monographs in Allergy 4:69–147
    [Google Scholar]
  21. Warren L. 1960; Thiobarbituric acid spray reagent for deoxy sugars and sialic acids. Nature: London; 186 237
    [Google Scholar]
  22. Weissbach A., Hurwitz J. 1959; The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. Journal of Biological Chemistry 234:705–709
    [Google Scholar]
  23. Wenzel F. J., Emanuel D. A., Gray R. L. 1971; Immunofluorescent studies in patients with farmer’s lung. Journal of Allergy & Clinical Immunology 48:224–229
    [Google Scholar]
  24. Wenzel F. J., Gray R. L., Roberts R. C., Emanuel D. A. 1974; Serologic studies in farmer’s lung. American Review of Respiratory Disease 109:464–468
    [Google Scholar]
  25. Westphal O., Jann K. 1965; Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure. In Methods in Carbohydrate Chemistry 5 pp. 83–91 Whistler R. L., Wolfron M. L. Edited by New York: Academic Press;
    [Google Scholar]
  26. Westphal O., Lüderitz O. 1954; Chemische Erforschung von Lipopolysacchariden der Gramnegativen Bakterien. Angewandte Chemie 66:407–417
    [Google Scholar]
  27. Zey P., Jackson S. 1973; Conditions that affect the colorimetric analysis of lipopolysaccharide from Escherichia coli and Treponema pallidum. Applied Microbiology 26:129–133
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-86-2-250
Loading
/content/journal/micro/10.1099/00221287-86-2-250
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error