SUMMARY: Extracts were prepared from several strains of and spp. Esterases, NADP-dependent isocitrate de-hydrogenase and malate dehydrogenase activities were assayed after electrophoresis of extracts of polyacrylamide gels. Except for and which resolved activity bands for the acetate-esterase only, the remaining bacteria exhibited species-specific esterase patterns also for the propionate and butyrate substrates. The multiple esterase patterns from ATCC25238 were qualitatively and quantitatively different from those of ATCC23246. This finding and other evidence supports a taxonomic shift of the latter to a species level of that genus. The atypical neisserias and appeared to exhibit an intrageneric specificity in their esterase patterns with those from but not to the other spp. tested. The malate dehydrogenase patterns from the atypical neisserias and ATCC23246 were qualitatively similar; however, the patterns of isocitrate dehydrogenase activity were variable for these species. was distinct in its esterase and dehydrogenase bands, strongly suggesting its taxon unrelatedness to the genus or the atypical neisserias. Of the enzymes assayed, esterase proved to be the most reliable for taxonomic identifications.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error