1887

Abstract

SUMMARY: The production of enterotoxins, lipase and total extracellular protein by four strains of grown in batch culture at a controlled pH of 6·5 in a completely defined medium was markedly reduced by glucose or glycerol constantly maintained at 0·1. A concomitant increase in the production of deoxy-ribonuclease, up to 13-fold, showed however that not all extracellular proteins are under the same control mechanism. The presence of glucose and glycerol in the medium also resulted in a rapid increase in the specific growth rate. However, growth of s6 in Mg-limited continuous culture showed that glucose repression of enterotoxin B when the growth rate was held constant was more than twice that in batch culture. Therefore glucose repression can occur independently of an increase in growth rate. The specific rate of production of enterotoxin B, lipase, deoxyribonuclease, -haemolysin and total extracellular protein by s6 increased as the growth rate increased from 0·07 to 0·24 h. Non-replicating cells grown in the absence of glucose produced considerable amounts of enterotoxin, and production was not repressed by the presence of glucose in the re-suspension medium. In contrast, no enterotoxin B or C was obtained from non-replicating cells grown in the presence of glucose. Chloramphenicol completely inhibited enterotoxin production by non-replicating cells, indicating that synthesis of new protein was required.

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/content/journal/micro/10.1099/00221287-86-1-75
1975-01-01
2022-01-27
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References

  1. Blumenthal H. V. 1972; Glucose catabolism in staphyloccocci. In The Staphylococci pp. 113–135 Cohen J. A. Edited by New York: John Wiley;
    [Google Scholar]
  2. Chesbro W. R., Heydrick F. P., Martineau R., Perkins G. N. 1965; Purification of staphylococcal β-haemolysin and action on staphylococcal and streptococcal cell walls. Journal of Bacteriology 89:378–389
    [Google Scholar]
  3. Coleman G. 1967; Studies on the regulation of extracellular enzyme formation by Bacillus subtilis. Journal of General Microbiology 49:421–431
    [Google Scholar]
  4. Czop J. K., Bergdoll M. S. 1974; Staphylococcal enterotoxin synthesis during the exponential, transitional, and stationary growth phases. Infection and Immunity 9:229–235
    [Google Scholar]
  5. De Crombrugghie B., Perlman R. L., Varmus H. E., Pastan I. 1969; Regulation of inducible enzyme synthesis in Escherichia coli by cyclic adenosine 3′,5′-monophosphate. Journal of Biological Chemistry 244:5828–5835
    [Google Scholar]
  6. Dornbusch K., Hallander H. O. 1973; Transduction of penicillinase production and methicillin resistance - enterotoxin B production in strains of Staphylococcus aureus. Journal of General Microbiology 76:1–11
    [Google Scholar]
  7. Eagon R. G. 1968 Advances in General Microbiology Methods Minneapolis: Burgess Publishing Co;
    [Google Scholar]
  8. Forsgren A., Nordström K., Philipson L., Sjöquist J. 1971; Protein A mutants of Staphylococcus aureus. Journal of Bacteriology 107:245–250
    [Google Scholar]
  9. Gallo M., Katz E. 1972; Regulation of secondary metabolite biosynthesis: catabolite repression of phenoxazinone synthase and actinomycin formation by glucose. Journal of Bacteriology 109:659–667
    [Google Scholar]
  10. Herbert D., Elsworth R., Telling R. C. 1956; The continuous culture of bacteria; a theoretical and experimental study. Journal of General Microbiology 14:601–622
    [Google Scholar]
  11. Jarvis A. W., Lawrence R. C., Pritchard G. G. 1973; Production of staphylococcal enterotoxins A, B and C under conditions of controlled pH and aeration. Infection and Immunity 7:847–854
    [Google Scholar]
  12. Mandelstam J. 1962; The repression of constitutive β-galactosidase in Escherichia coli by glucose and other carbon sources. Biochemical Journal 82:489–493
    [Google Scholar]
  13. Markus Z., Silverman G. J. 1969; Enterotoxin B synthesis by replicating and non-replicating cells of Staphylococcus aureus. Journal of Bacteriology 97:506–512
    [Google Scholar]
  14. Metzger J. F., Johnson A. D., Collins W. S. II Mcgann V. 1973; Staphylococcus aureus enterotoxin B release (excretion) under controlled conditions of fermentation. Applied Microbiology 25:770–773
    [Google Scholar]
  15. Miller R. D., Fung D. Y. C. 1973; Amino-acid requirements for the production of enterotoxin B by Staphylococcus aureus. Applied Microbiology 25:800–806
    [Google Scholar]
  16. Morse S. A., Baldwin J. N. 1971; Regulation of staphylococcal enterotoxin B: effect of thiamine starvation. Applied Microbiology 22:242–249
    [Google Scholar]
  17. Morse S. A., Mah R. A., Dobrogosz W. J. 1969; Regulation of staphylococcal enterotoxin B. Journal of Bacteriology 98:4–9
    [Google Scholar]
  18. Omenn G. S., Friedman J. 1970; Isolation of mutants of Staphylococcus aureus lacking extracellular nuclease activity. Journal of Bacteriology 101:921–924
    [Google Scholar]
  19. Paigen K., Williams B. 1970; Catabolite repression and other control mechanisms in carbohydrate utilization. In Advances in Microbial Physiology 4 pp. 251–324 Rose A. H., Wilkinson J. F. Edited by London: Academic Press;
    [Google Scholar]
  20. Pirt S. J. 1969; Microbial growth and product formation. In Microbial growth pp. 199–221 Meadow P. M., Pirt S. J. Edited by Cambridge: Cambridge University Press;
    [Google Scholar]
  21. Rotham-Denes L. B., Hesse J. E., Epstein H. 1973; Role of cyclic adenosine 3′ 5′-monophosphate in the in vivo expression of the galactose operon of Escherichia coli. Journal of Bacteriology 114:1040–1044
    [Google Scholar]
  22. Schaechter M., Maaløe O., Kjeldgaard N. O. 1958; Dependency on medium and temperature of cell size and chemical composition during balanced growth of Salmonella typhimurium. Journal of General Microbiology 19:592–606
    [Google Scholar]
  23. Stanier R. Y., Doudoroff M., Adelberg E. A. 1971 General Microbiology, 3rd ed. London: Macmillan;
    [Google Scholar]
  24. Tanaka S., Iuchi S. 1971; Induction and repression of an extracellular protease of Vibrio parahaemolyticus. Biken Journal 14:81–96
    [Google Scholar]
  25. Wu C. -H., Bergdoll M. S. 1971; Stimulation of enterotoxin B production. II. Synthetic medium for staphylococcal growth and enterotoxin B production. Infection and Immunity 3:784–792
    [Google Scholar]
  26. Yano T., Koga S. 1973; Dynamic behaviour of the chemostat subject to product inhibition. Journal of General and Applied Microbiology 19:97–114
    [Google Scholar]
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