RT Journal Article SR Electronic(1) A1 Sietsma, J. H. A1 Child, J. J. A1 Nesbitt, L. R. A1 Haskins, R. H.YR 1975 T1 Chemistry and Ultrastructure of the Hyphal Walls of Pythium Acanthicum JF Microbiology, VO 86 IS 1 SP 29 OP 38 DO https://doi.org/10.1099/00221287-86-1-29 PB Microbiology Society, SN 1465-2080, AB SUMMARY: Combined methylation, Smith degradation and enzymic studies show that the hyphal wall of Pythium consists predominantly of a (1,3)-linked β-d-glucan main chain substituted by a short chain at the 6-position of each third unit. Electron micrographs show that the hyphal wall has two different textures; the inner surface is distinctly microfibrillar while the outer surface is non-fibrillar. The microfibrils are covered with amorphous matrix material, partly soluble in aqueous KOH and almost completely removable by treatment with exo-laminaranase. This matrix material consists also of a branched β-d-glucan containing (1,3)- and (1,6)-linkages. The microfibrils remaining after (1,3)-glucanase treatment are composed of a β-glucan with (1,4)- and (1,3)-linked units that are occasionally substituted in the 6-positions., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-86-1-29