SUMMARY: Combined methylation, Smith degradation and enzymic studies show that the hyphal wall of Pythium consists predominantly of a (1,3)-linked β-D-glucan main chain substituted by a short chain at the 6-position of each third unit. Electron micrographs show that the hyphal wall has two different textures; the inner surface is distinctly microfibrillar while the outer surface is non-fibrillar. The microfibrils are covered with amorphous matrix material, partly soluble in aqueous KOH and almost completely removable by treatment with exo-laminaranase. This matrix material consists also of a branched β-D-glucan containing (1,3)- and (1,6)-linkages. The microfibrils remaining after (1,3)-β-glucanase treatment are composed of a β-glucan with (1,4)- and (1,3)-linked units that are occasionally substituted in the 6-positions.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error