1887

Abstract

SUMMARY: Combined methylation, Smith degradation and enzymic studies show that the hyphal wall of Pythium consists predominantly of a (1,3)-linked β-D-glucan main chain substituted by a short chain at the 6-position of each third unit. Electron micrographs show that the hyphal wall has two different textures; the inner surface is distinctly microfibrillar while the outer surface is non-fibrillar. The microfibrils are covered with amorphous matrix material, partly soluble in aqueous KOH and almost completely removable by treatment with exo-laminaranase. This matrix material consists also of a branched β-D-glucan containing (1,3)- and (1,6)-linkages. The microfibrils remaining after (1,3)-β-glucanase treatment are composed of a β-glucan with (1,4)- and (1,3)-linked units that are occasionally substituted in the 6-positions.

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/content/journal/micro/10.1099/00221287-86-1-29
1975-01-01
2019-10-14
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-86-1-29
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