1887

Abstract

SUMMARY: Combined methylation, Smith degradation and enzymic studies show that the hyphal wall of Pythium consists predominantly of a (1,3)-linked --glucan main chain substituted by a short chain at the 6-position of each third unit. Electron micrographs show that the hyphal wall has two different textures; the inner surface is distinctly microfibrillar while the outer surface is non-fibrillar. The microfibrils are covered with amorphous matrix material, partly soluble in aqueous KOH and almost completely removable by treatment with exo-laminaranase. This matrix material consists also of a branched --glucan containing (1,3)- and (1,6)-linkages. The microfibrils remaining after (1,3)-glucanase treatment are composed of a -glucan with (1,4)- and (1,3)-linked units that are occasionally substituted in the 6-positions.

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1975-01-01
2021-05-11
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