1887

Abstract

Glutamate--ketoadipate transaminase, saccharopine reductase, and saccharopine dehydrogenase activities were demonstrated in extracts of but -aminoadipate reductase activity could not be measured in whole cells or in extracts. Lysine auxotroph grew in the presence of -lysine or --aminoadipate and incorporated radioactivity from --amino-[C]adipate into lysine during growth. Growing wild-type cells converted -[U-C]lysine into -amino-[C]adipate, suggesting both biosynthetic and degradative roles for -aminoadipate. Lysine auxotrophs and of unlike lysine auxotrophs of satisfied their growth requirement with -pipecolate. Moreover, extracts of wild-type catalysed the conversion of -pipecolate to -aminoadipate--semialdehyde. These results suggest a bio-synthetic role for -pipecolate in but not in

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1975-01-01
2021-08-03
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