1887

Abstract

Summary: A mutant of which required riboflavin for growth was grown with limiting or excess riboflavin. Riboflavin deficiency decreased the content of flavin, particularly of FMN in the particulate fraction. The activities of particulate succinate and NADH dehydrogenases also decreased, whereas α-glycerophosphate dehydrogenase activity did not alter. When riboflavin was added back to a starved culture, the bacterial flavin content recovered quickly, even when chloramphenicol was present. Succinate dehydrogenase activity also recovered, but this increase was inhibited by chloramphenicol.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-85-2-303
1974-12-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/85/2/mic-85-2-303.html?itemId=/content/journal/micro/10.1099/00221287-85-2-303&mimeType=html&fmt=ahah

References

  1. Bacher A., Eggers U., Lingens F. 1973; Genetic control of riboflavin synthetase in Bacillus subtilis. Archiv fur Mikrobiologie 89:73–77
    [Google Scholar]
  2. Burch H. B. 1957; Fluorimetric assay of FMN and FAD. Methods in Enzymology 3:960–962
    [Google Scholar]
  3. Chaix P., Petit J. F. 1956; Etude de differents spectres cytochromiques de Bacillus subtilis. Biochimica et biophysica acta 22:66–71
    [Google Scholar]
  4. Chang J. P., Lascelles J. 1963; Nitrate reductase in cell-free extracts of a haem-requiring strain of Staphylococcus aureus. Biochemical Journal 89:503–510
    [Google Scholar]
  5. Clegg R. A., Skyrme J. E. 1973; The effects of iron-limited growth on the reduced nicotinamide adenine dinucleotide dehydrogenase activity and the membrane proteins of Candida utilis mitochondria. Biochemical Journal 136:1029–1037
    [Google Scholar]
  6. Dietrich J., Henning U. 1970; Regulation of pyruvate dehydrogenase complex synthesis in Escherichia coli K12. Identification of the inducing metabolite. European Journal of Biochemistry 14:258–269
    [Google Scholar]
  7. Farrand S. K., Taber H. W. 1973; Physiological effects of menaquinone deficiency in Bacillus subtilis. Journal of Bacteriology 115:1035–1044
    [Google Scholar]
  8. Fortnagel P., Freese E. 1968; Analysis of sporulation mutants. II. Mutants blocked in the citric acid cycle. Journal of Bacteriology 95:1431–1438
    [Google Scholar]
  9. Gibson F., Cox G. B. 1973; The use of mutants of Escherichia coli K12 in studying electron transport and oxidative phosphorylation. Essays in Biochemistry 9:1–29
    [Google Scholar]
  10. Haddock B. A. 1973; The reconstitution of oxidase activity in membranes derived from a 5-aminolaevulinic acid-requiring mutant of Escherichia coli. Biochemical Journal 136:877–884
    [Google Scholar]
  11. Haddock B. A., Shairer H. U. 1973; Electron transport chains of Escherichia coli. Reconstitution of respiration in a 5-aminolaevulinic acid-requiring mutant. European Journal of Biochemistry 35:34–45
    [Google Scholar]
  12. Kogut M., Lightbown J. W. 1962; Selective inhibition by 2-heptyl-4-hydroxyquinoline-N-oxide of certain oxidation-reduction reactions. Biochemical Journal 84:368–382
    [Google Scholar]
  13. Light P. A. 1972a; Influence of environment on mitochondrial function in yeast. Journal of Applied Chemistry and Biotechnology 22:509–526
    [Google Scholar]
  14. Light P. A. 1972b; Mitochondrial effects of copper-limited growth of Torulopsis utilis. FEBS Letters 19:319–322
    [Google Scholar]
  15. Light P. A., Garland P. B. 1971; A comparison of mitochondria from Torulopsis utilis grown in continuous culture with glycerol, iron, ammonium, magnesium or phosphate as the limiting nutrient. Biochemical Journal 124:123–134
    [Google Scholar]
  16. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  17. Miki K., Sekuzu I., Okunuki K. 1967; Cytochromes of Bacillus subtilis. I. Cytochrome system in the particulate fraction. Report of Scientific Works. Faculty of Science, Osaka University 15:33–58
    [Google Scholar]
  18. Mindich L. 1970; Membrane synthesis in Bacillus subtilis. I. Isolation and properties of strains bearing mutations in glycerol metabolism. Journal of Molecular Biology 49:415–432
    [Google Scholar]
  19. Ohne M., Rutberg B., Hoch J. A. 1973; Genetic and biochemical characterization of mutants of Bacillus subtilis deficient in succinate dehydrogenase. Journal of Bacteriology 115:738–745
    [Google Scholar]
  20. Ornston L. N., Stanier R. Y. 1966; The conversion of catechol and protocatechuate to //-ketoadipate by Pseudomonas putida. I. Biochemistry. Journal of Biological Chemistry 241:3776–3786
    [Google Scholar]
  21. Tochikubo K. 1971; Changes in terminal respiratory pathways of Bacillus subtilis during germination, outgrowth and vegetative growth. Journal of Bacteriology 108:652–661
    [Google Scholar]
  22. Wilson A. C., Pardee A. B. 1962; Regulation of flavin synthesis by Escherichia coli. Journal of General Microbiology 28:283–303
    [Google Scholar]
  23. Yang C. C., Legallais V. 1954; A rapid and sensitive recording spectrophotometer for the visible and ultraviolet region. I. Description and performance. Reviews of Scientific Instruments 25:801–807
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-85-2-303
Loading
/content/journal/micro/10.1099/00221287-85-2-303
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error