1887

Abstract

Summary: The technique of autoradiography in the electron microscope has been used to determine the site of incorporation of tracer from tritium-labelled glucose, amino acids and bacteria by and the ovine form of . Both protozoa incorporated tracer from glucose principally into protozoal polysaccharide and intracellular bacteria. The results with the bacteria confirmed previous findings that digested rapidly in vesicles in the endoplasm, whereas spp. lysed the bacteria in the medium before incorporation. These products were then taken up both by the protozoa and the bacteria attached to their surface. In the presence of label from tritiated was found principally in the endoplasm, whereas that from glycine was present in the ectoplasm also.

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/content/journal/micro/10.1099/00221287-85-2-265
1974-12-01
2021-07-24
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References

  1. Caro L. G., van Tubergen R., Kolb J. A. 1962; High resolution autoradiography. 1. Methods. Journal of Cell Biology 15:173–188
    [Google Scholar]
  2. Coleman G. S. 1960; Maintenance of oligotrich protozoa from the sheep rumen in vitro. Journal of General Microbiology 22:555–563
    [Google Scholar]
  3. Coleman G. S. 1963; The growth and metabolism of rumen ciliate protozoa. Symposia of the Society for General Microbiology 13:298–325
    [Google Scholar]
  4. Coleman G. S. 1964; The metabolism of Escherichia coli and other bacteria by Entodinium caudatum. Journal of General Microbiology 37:209–223
    [Google Scholar]
  5. Coleman G. S. 1967; The metabolism of free amino acids by washed suspensions of the rumen ciliate Entodinium caudatum. Journal of General Microbiology 47:433–447
    [Google Scholar]
  6. Coleman G. S. 1969; The metabolism of starch, maltose, glucose and some other sugars by the rumen ciliate Entodinium caudatum. Journal of General Microbiology 57:303–332
    [Google Scholar]
  7. Coleman G. S. 1972; The metabolism of starch, glucose, amino acids, purines, pyrimidines and bacteria by the rumen ciliate Entodinium simplex. Journal of General Microbiology 71:117–131
    [Google Scholar]
  8. Coleman G. S., Hall F. J. 1969; Electron microscopy of the rumen ciliate Entodinium caudatum, with special reference to the engulfment of bacteria and other particulate matter. Tissue and Cell 1:607–618
    [Google Scholar]
  9. Coleman G. S., Hall F. J. 1971; A study in the light and electron microscope of the extruded peristome and related structures of the rumen ciliate Entodinium caudatum. Tissue and Cell 3:371–380
    [Google Scholar]
  10. Coleman G. S., Hall F. J. 1972; Fine structural studies on the digestion of bacterial species in the rumen ciliate Entodinium caudatum. Tissue and Cell 4:37–48
    [Google Scholar]
  11. Coleman G. S., Laurie J. I. 1974a; The metabolism of starch, glucose, amino acids, purines, pyrimidines and bacteria by three Epidinium spp. isolated from the rumen. Journal of General Microbiology 85:244–256
    [Google Scholar]
  12. Coleman G. S., Laurie J. I. 1974b; The utilization of Bacillus megaterium and the release of a lytic enzyme by three Epidinium spp. isolated from the rumen. Journal of General Microbiology 85:257–264
    [Google Scholar]
  13. Roberts R. B., Abelson P. H., Cowie D. E., Bolton E. T., Britten R. J. 1955; Studies on biosynthesis in Escherichia coli. Publications of the Carnegie Institution no 607
    [Google Scholar]
  14. White R. W. 1969; Viable bacteria inside the rumen ciliate Entodinium caudatum. Journal of General Microbiology 56:403–408
    [Google Scholar]
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