Summary: Washed suspensions of three spp., grown and incubated anaerobically in salts solution, released into the medium protein containing hexokinase and an enzyme which lysed and Material from the lysate, containing bound amino acids, was incorporated by spp. at a rate that would allow only very slow growth if it were the sole source of nitrogen. Although there was no massive cell lysis the enzymes were liberated as the result of a release of whole cell contents by an unknown mechanism. In the presence of other rumen protozoa, protein, but no lytic enzyme, appeared in the medium. No good evidence was obtained for the existence in the rumen of a lytic enzyme associated with the presence of spp.


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