The Metabolism of Starch, Glucose, Amino Acids, Purines, Pyrimidines and Bacteria by Three & spp. Isolated from the Rumen Free

Abstract

Summary: Washed suspensions of spp. grown and incubated anaero-bically in salts medium engulfed all the bacteria tested at rates ranging from 60 to 7000/protozoon/h at a density of 10/ml. The rate of uptake of bacteria was also compared by the volume of medium cleared of bacteria/h. On both bases was taken up most rapidly. Some of the bacteria were killed and digested inside the protozoa. The spp. took up a wide range of amino acids at rates varying from 6 to 250 nmol/10 protozoa/h and incorporated them into protein, mostly without conversion to other amino acids. The protozoa also incorporated purines and pyrimidines into nucleic acid and interconverted adenine and guanine and also uracil and cytosine.

took up [C]gIucose more rapidly than and used the glucose carbon for the synthesis of an intracellular glucose polysaccharide and at least eight amino acids, 15 % of the C in the protozoa appearing in protein. The protozoa also synthesized protein from starch grains. Evidence has been obtained that cannot obtain all the protein it requires for growth from bacteria, free amino acids, glucose or starch and must incorporate protein from the wholemeal flour on which it is grown.

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1974-12-01
2024-03-28
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References

  1. Bailey R. W., Gaillard B. D. E. 1965; Carbohydrases of the rumen ciliate Epidinium ecaudatum (Crawley). Hydrolysis of plant hemicellulose fractions and //-linked glucose polymers. Biochemical Journal 95:758–766
    [Google Scholar]
  2. Bird P. R. 1972; Sulphur metabolism and excretion studies in ruminants. 6. The digestibility and utilization by sheep of 35S from 35S-labelled ruminal microorganisms. Australian Journal of Biological Science 25:195–203
    [Google Scholar]
  3. Clarke R. T. J. 1964; Ciliates of the rumen of domestic cattle (Bos taurus L.). New Zealand Journal of Agricultural Research 7:248–257
    [Google Scholar]
  4. Coleman G. S. 1956; The dissimilation of amino acids by Rhodospirillum rubrum. Journal of General Microbiology 15:248–256
    [Google Scholar]
  5. Coleman G. S. 1964; The metabolism of Escherichia coli and other bacteria by Entodinium caudatum. Journal of General Microbiology 37:209–223
    [Google Scholar]
  6. Coleman G. S. 1967a; The metabolism of free amino acids by washed suspensions of the rumen ciliate Entodinium caudatum. Journal of General Microbiology 47:433–447
    [Google Scholar]
  7. Coleman G. S. 1967b; The metabolism of the amino acids of Escherichia coli and other bacteria by the rumen ciliate Entodinium caudatum. Journal of General Microbiology 47:449–464
    [Google Scholar]
  8. Coleman G. S. 1968; The metabolism of bacterial nucleic acid and of free components of nucleic acid by the rumen ciliate Entodinium caudatum. Journal of General Microbiology 54:83–96
    [Google Scholar]
  9. Coleman G. S. 1969 a; The cultivation of the rumen ciliate Entodinium simplex. Journal of General Microbiology 57:81–90
    [Google Scholar]
  10. Coleman G. S. 1969b; The metabolism of starch, maltose, glucose and some other sugarsby the rumen ciliate Entodinium caudatum. Journal of General Microbiology 57:303–332
    [Google Scholar]
  11. Coleman G. S. 1972; The metabolism of starch, glucose, amino acids, purines, pyrimidines and bacteria by the rumen ciliate Entodinium simplex. Journal of General Microbiology 71:117–131
    [Google Scholar]
  12. Coleman G. S. 1973; The metabolism of bacteria and amino acids by three Epidinium spp. isolated from the rumen and cultured in vitro. Journal of General Microbiology 77:viii
    [Google Scholar]
  13. Coleman G. S., Davies J. I., Cash M. A. 1972; The cultivation of the rumen ciliates Epidinium ecaudatum caudatum and Polyplastron multivesiculatum in vitro. Journal of General Microbiology 73:509–521
    [Google Scholar]
  14. Coleman G. S., Hall F. J. 1974; The metabolism of Epidinium ecaudatum caudatum and Entodinium caudatum as shown by autoradiography in the electron microscope. Journal of General Microbiology 85:265–273
    [Google Scholar]
  15. Coleman G. S., Laurie J. I. 1974; The utilization of Bacillus megaterium and the release of a lytic enzyme by three Epidinium spp. isolated from the rumen. Journal of General Microbiology 85:257–264
    [Google Scholar]
  16. Conway E. J. 1947 Microdiffusion Analysis and Volumetric Error London: Crosby Lockwood & Son;
    [Google Scholar]
  17. Curds C. R., Cockburn A. 1968; Studies on the growth and feeding of Tetrahymena pyriformis in axenic and monoxenic culture. Journal of General Microbiology 54:343–358
    [Google Scholar]
  18. Eadie J. M. 1962; Inter-relationships between certain rumen ciliate protozoa. Journal of General Microbiology 29:579–588
    [Google Scholar]
  19. Eadie J. M. 1967; Studies on the ecology of certain rumen ciliate protozoa. Journal of General Microbiology 49:175–194
    [Google Scholar]
  20. Eadie J. M., Mann S. O. 1970; Development of the rumen microbial population: high starch diets and instability. In Physiology of Digestion and Metabolism in the Ruminant pp. 335–347 Newcastle-upon-Tyne: Oriel Press;
    [Google Scholar]
  21. Florkin M., Duchateau-Bosson G. 1943; Forms of uricolytic enzyme systems and the evolution of purine metabolism in the animal kingdom. Archives Internationales de physiologie 53:267–307
    [Google Scholar]
  22. Gutierrez J., Davis R. E. 1962; Culture and metabolism of the rumen ciliate Epidinium ecaudatum (Crawley). Applied Microbiology 10:305–308
    [Google Scholar]
  23. Heal O. W. 1967; Quantitative studies on soil amoebae. In Progress in Soil Biology pp. 120–126 Edited by Graff O., Satchell J. E. Amsterdam: North Holland Publishing Co;
    [Google Scholar]
  24. Lowry O. M., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  25. Oxford A. E. 1959; Bloat in cattle. 15. Further observations concerning the ciliate Epidinium ecaudatum, an inhabitant of the rumens of cows liable to legume bloat. New Zealand Journal of Agricultural Research 2:365–374
    [Google Scholar]
  26. Proper G., Garver J. C. 1966; Mass culture of the protozoa Colpoda steinii. Biotechnology and Bioengineering 8:287–296
    [Google Scholar]
  27. White R. W. 1969; Viable bacteria inside the rumen ciliate Entodinium caudatum. Journal of General Microbiology 56:403–408
    [Google Scholar]
  28. Wright D. E., Hungate R. E. 1967; Amino acid concentrations in rumen fluid. Applied Microbiology 15:148–151
    [Google Scholar]
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