1887

Abstract

Summary: Mutuants of were induced by u.v. light and selected by the criterion that they formed small colonies on a nutrient agar at normal temperature. Nine slow-growing mutant strains were isolated. These mutants were characterized and compared during exponential growth in a nutrient medium with respect to doubling time, average cell mass, and DNA and RNA contents. The mutants showed only two groupings of doubling times, approximately 60 min and 35 min, as compared with 20 min for the parent. The Mutants were heterogeneous with regard cell mass, ranging from considerably larger than the parent to smaller. There was no constancy between average cell mass and the population doubling time. RNA:mass and DNA:mass ratios were similar among mutant strains with the same doubling time despite large variations in average cell mass.

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/content/journal/micro/10.1099/00221287-85-2-203
1974-12-01
2021-10-18
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References

  1. Adelberg E. A. 1966 Papers on Bacterial Genetics Boston: Little, Brown;
    [Google Scholar]
  2. Bockrath R. C., Osborn M., Person S. 1968; Nonsense suppression in a multiauxotrophic derivative of Escherichia coli 15T: identification and consequences of an amber triplet in the deoxyribomutase gene. Journal of Bacteriology 96:146–164
    [Google Scholar]
  3. Cooper S., Helmstetter C. E. 1968; Chromosome replication and the division cycle of Escherichia coli B/r. Journal of Molecular Biology 31:519–540
    [Google Scholar]
  4. Forchhammer J., Lindahl L. 1971; Growth rate of polypeptide chains as a function of cell growth rate in a mutant of Escherichia coli 15. Journal of Molecular Biology 55:563–568
    [Google Scholar]
  5. Helmstetter C. E. 1969; Sequence of bacterial reproduction. Annual Review of Microbiology 23:223–234
    [Google Scholar]
  6. Koch A. L. 1970; Turbidity measurements of bacterial cultures in available commercial instruments. Analytical Biochemistry 38:252–259
    [Google Scholar]
  7. Kubitschek H. E., Freedman M. L. 1971; Chromosome replication and the division cycle of Escherichia coli B/r. Journal of Bacteriology 107:95–99
    [Google Scholar]
  8. Maaløe O., Kjeldgaard N. O. 1966 Control of Macromolecular Synthesis New York: W. A. Benjamin;
    [Google Scholar]
  9. Person S., Bockrath R. C. 1964; Differential mutation produced by the decay of incorporated tritium compounds in E. coli. Biophysical Journal 4:355–365
    [Google Scholar]
  10. Schaechter M., Maaloe O., Kjeldgaard N. O. 1958; Dependency on medium and temperature of cell size and chemical composition during balanced growth of Salmonella typhimurium. Journal of General Microbiology 19:592–606
    [Google Scholar]
  11. Schmidt G., Thannhauser S. J. 1945; A method for the determination of desoxyribonucleic acid, ribonucleic acid and phosphoproteins in animal tissue. Journal of Biological Chemistry 161:83–89
    [Google Scholar]
  12. Zaritsky A., Pritchard R. H. 1973; Changes in cell size and shape associated with changes in the replication time of the chromosome of Escherichia coli. Journal of Bacteriology 114:824–837
    [Google Scholar]
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