@article{mbs:/content/journal/micro/10.1099/00221287-85-2-190, author = "Dabbagh, R. and Conant, K. F. and Burns, R. O.", title = "Effect of Temperature on Saprophytic Cryptococci: Observations Relating to Wall Biosynthesis at Non-permissive Growth Temperatures", journal= "Microbiology", year = "1974", volume = "85", number = "2", pages = "190-202", doi = "https://doi.org/10.1099/00221287-85-2-190", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-85-2-190", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Summary: An attempt was made to define the basis of temperature-induced aberrant wall biosynthesis in Cryptococcus diffluens. A quantitative determination of the sulphydryl content of the cell surface of C. diffluens as an index of a protein disulphide reductase activity failed to reveal differences between cells cultured at permissive and non-permissive growth temperatures. Assays of β-1,3-exo-glucanase showed no abnormal activities which could be responsible for the temperature-induced aberrations. Cryptococcus diffluens excreted low molecular weight compounds non-specifically: UMP and AMP were the predominant nucleotides excreted. Uridine diphosphoglucose, a precursor of glucan biosynthesis, was accumulated and excreted by C. diffluens at non-permissive but not at permissive growth temperatures. A correlation exists between the rate of yeast multiplication at permissive temperatures and the extent of residual growth upon subsequent exposure to a non-permissive temperature.", }