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Abstract
SUMMARY: Fourteen mutants of Pseudomonas aeruginosa ATCC 17423, isolated for their inability to grow rapidly on heptane while still growing normally on heptanoic acid, were all also able to grow on heptanol or heptanal. A study of heptane oxidation in extracts showed differences between the mutants, which were resolved into four groups (A to D). Group A or B alone could not oxidize heptane, but a mixture of the extracts was effective, and either A or B stimulated the slight activity of extracts from group D mutants. Extracts from group C mutants were inactive in virtually all circumstances. The mutants therefore lack, in different ways, one or more of the three components demonstrated to be necessary for heptane oxidation, but no mutant totally lacking rubredoxin was demonstrated.
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