SUMMARY: The metabolic control of the protocatechuate branch of the 3-oxoadipate pathway in was examined and the specific inducers identified using appropriately blocked mutants. Three successive inductive events permitted the synthesis of the five enzymes converting -hydroxybenzoate to 3-oxoadipyl-CoA: the independent induction of 4-hydroxybenzoate 3-mono-oxygenase by its own specific substrate, the independent induction of protocatechuate 3,4-dioxygenase by either protocatechuate or -hydroxybenzoate, and finally the co-ordinate induction of 3-carboxymuconate cyclase, 3-carboxymucono-lactone hydrolase and 3-oxoadipate CoA-transferase by cither protocatechuate or -hydroxybenzoate. Evidence is presented which suggests that is unable to metabolize catechol or its usual precursors, and the significance of this is discussed in relation to control of the protocatechuate branch and the differing control mechanisms governing the synthesis of enzymes of the 3-oxoadipate pathway in other fungi and bacteria.


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