1887

Abstract

Summary: Four toxigenic strains each of and were grown in Schmid peptone--glucose medium to determine their ability to carry out nitrification and in a yeast extract--sucrose (YES) medium to test their toxigenicity. Two nitrifying strains each of and were grown in YES medium to examine their ability to form aflatoxin. A steam-distillation method was used to determine ammonium-, nitrite- and nitrate-nitrogen, whereas thin-layer chromatography and fluorometry served to measure aflatoxin content of broths. All eight toxigenic strains produced nitrate in Schmid and YES media when incubated without shaking. Shaking of cultures tended to increase the amount of nitrate formed in Schmid but not in YES medium. Amounts of aflatoxin produced varied with media and methods of incubation. All four nitrifying strains formed aflatoxin in YES medium. Toxigenic and nitrifying strains that gave high yields of aflatoxin did not always produce high levels of nitrate.

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/content/journal/micro/10.1099/00221287-84-2-357
1974-10-01
2022-01-23
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References

  1. Aleem M. I, Lees H., Lyric R. 1964; Ammonium oxidation by cell-free extracts of Aspergillus wentii . Canadian Journal of Biochemistry 42:989–998
    [Google Scholar]
  2. Bremner J. M. 1965; Inorganic forms of nitrogen. In Methods of Soil Analysis. Part 2. Chemical and microbiological properties Madison: American Society of Agronomics;
    [Google Scholar]
  3. Bremner J. M., Keeney D. R. 1965; Steam distillation methods for determination of ammonium, nitrate and nitrite. Analytica chimica acta 32:485–495
    [Google Scholar]
  4. Davis N. D., Diener U. L., Eldridge D. W. 1966; Production of aflatoxin Bx and Gi by Aspergillus flavus in a semisynthetic medium. Applied Microbiology 14:378–380
    [Google Scholar]
  5. Detroy R. W., Hesseltine C. W. 1969; Net synthesis of 14C-labeled lipids and aflatoxins in resting cells of Aspergillus parasiticus . Developments in Industrial Microbiology 10:127–133
    [Google Scholar]
  6. Detroy R. W., Lillehoj E. B., Ciegler A. 1971; Aflatoxin and related compounds. In Microbial Toxins vol. 6 Edited by Ciegler A., Kadis S., Ajl S. J. New York: Academic Press;
    [Google Scholar]
  7. Doxtader K. G., Alexander M. 1966; Role of 3-nitropropionic acid in nitrate formation by Aspergillus flavus . Journal of Bacteriology 91:1186–1191
    [Google Scholar]
  8. Eylar O. R., Schmidt E. L. 1959; A survey of heterotrophic micro-organisms from soil for ability to form nitrite and nitrate. Journal of General Microbiology 20:473–481
    [Google Scholar]
  9. Heimbrook M. E. 1969 Nitrification potential in the genus Aspergillus M.S. thesis University of Wisconsin; U.S.A.:
    [Google Scholar]
  10. Hora T. S., Iyengar M. R. S. 1960; Nitrification by soil fungi. Archiv fur Mikrobiologie 35:252–257
    [Google Scholar]
  11. Keeney D. R. 1970; Nitrates in plants and water. Journal of Milk and Food Technology 33:425–432
    [Google Scholar]
  12. Marshall K. C., Alexander M. 1961; Fungi active in heterotrophic nitrification. Canadian Journal of Microbiology 7:955–957
    [Google Scholar]
  13. Marshall K. C., Alexander M. 1962; Nitrification by Aspergillus flavus . Journal of Bacteriology 83:572–578
    [Google Scholar]
  14. Marth E. H. 1967; Aflatoxins and other mycotoxins in agricultural products. Journal of Milk and Food Technology 30:192–198
    [Google Scholar]
  15. Molina J. A. E., Alexander M. 1971; Formation of nitrate from 3-nitropropionate by Aspergillus flavus . Journal of Bacteriology 105:489–493
    [Google Scholar]
  16. Schmidt E. L. 1954; Nitrate formation by a soil fungus. Science, New York 119:187–189
    [Google Scholar]
  17. Schmidt E. L. 1960; Cultural conditions influencing nitrate formation by Aspergillus flavus . Journal of Bacteriology 79:553–557
    [Google Scholar]
  18. Shih C. N., Marth E. H. 1969; Improved procedures for measurement of aflatoxins with thin-layer chromatography and fluorometry. Journal of Milk and Food Technology 32:213–217
    [Google Scholar]
  19. Wogan G. N. 1966; Chemical nature and biological effects of the aflatoxins. Bacteriological Reviews 30:460–470
    [Google Scholar]
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