1887

Abstract

Summary: Three phages specific for bacteria harbouring the P-group plasmid RP1 have been isolated, and their properties compared with those of a previously described sex-specific phage, PRR1 (Olsen & Shipley, 1973). These phages are distinguishable from each other by various criteria, although in terms of host range to FP and RP lines of they comprise broadly two groups. Thus all the phages infect bacteria harbouring any of a group of plasmids with similar properties to those of RP1, but whereas the filamentous phage Pf3 is specific for this group, the host ranges of PRR1, PR3 and PR4 are considerably wider. Nevertheless, with one exception, this does not extend to plasmids isolated outside the United Kingdom, which suggests that all these plasmids share a common ancestry even though by other criteria they constitute three fairly discrete subgroups. Of the plasmids that fail to allow phage propagation, three, when present in the same cell as RP1, reduce its susceptibility to phage infection. This inhibition may reflect a relationship between these elements, similar to that found among plasmids of Enterobacteria. A correlation is observed between the susceptibility of bacteria to phage infection and their ability to mediate plasmid transfer, such that these phages can conveniently be used to isolate both derepressed or transfer-defective mutants of various R factors.

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/content/journal/micro/10.1099/00221287-84-2-332
1974-10-01
2022-06-29
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References

  1. Adams M. 1959 Bacteriophages New York: Interscience Publishing;
    [Google Scholar]
  2. Black W. A., Girdwood R. W. A. 1969; Carbenicillin resistant Pseudomonas aeruginosa . British Medical Journal iv:234
    [Google Scholar]
  3. Bradley D. E. 1974; Adsorption of bacteriophages specific for Pseudomonas aeruginosa R factors RPi and R1822. Biochemical and Biophysical Research Communications 57:893–900
    [Google Scholar]
  4. Bryan L. E., Semaka S. D., van de Elzen H. M., Kinnear J. E., Whttehouse R. L. S. 1973; Characteristics of R931 and other Pseudomonas aeruginosa R factors. Antibiotic Agents and Chemotherapy 3:625–637
    [Google Scholar]
  5. Chabbert Y. A., Scavizzi M. P., Witchitz J. L., Gerbaud G. R., Bouanchaud D. H. 1972; Incompatibility groups and the classification of fi~resistance factors. Journal of Bacteriology 112:666–675
    [Google Scholar]
  6. Chandler P. M., Krishnapillai V. 1974a; Phenotypic properties of R factors of Pseudomonas aeruginosa'. R factors readily transferable to Enterobacteriaceae . Genetical Research (in the Press)
    [Google Scholar]
  7. Chandler P. M., Krishnapillai V. 1974b; Phenotypic properties of R factors of Pseudomonas aeruginosa: R factors transferable only within Pseudomonas . Genetical Research (in the Press)
    [Google Scholar]
  8. Chandler P. M., Krishnapillai V. 1974c; Isolation and properties of recombination deficient mutants of Pseudomonas aeruginosa . Mutation Research 23:15–23
    [Google Scholar]
  9. Curtis N. A. C., Richmond M. H., Stanisich V. A. 1973; R-factor mediated resistance to penicillins which does not involve a β-lactamase. Journal of Bacteriology 79:163–165
    [Google Scholar]
  10. Datta N., Hedges R. W., Shaw E. J., Sykes R. B., Richmond M. H. 1971; Properties of an R factor from Pseudomonas aeruginosa . Journal of Bacteriology 108:1244–1249
    [Google Scholar]
  11. Datta N., Hedges R. W. 1972; Host range of R factors. Journal of General Microbiology 70:453–460
    [Google Scholar]
  12. Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. 1966; A proposal for uniform nomenclature in bacterial genetics. Genetics 54:61–76
    [Google Scholar]
  13. Edwards S., Meynell G. G. 1968; General method for isolating derepressed bacterial sex factors. Nature, London 219:869–870
    [Google Scholar]
  14. Grindley J. N., Anderson E. S. 1971; I-like resistance factors with the fi+ character. Genetical Research 17:267–271
    [Google Scholar]
  15. Grinsted J., Saunders J. R., Ingram L. C., Sykes R. B., Richmond M. H. 1972; Properties of an R factor which originated in Pseudomonas aeruginosa 1822. Journal of Bacteriology 110529–537
    [Google Scholar]
  16. Holloway B. W. 1969; Genetics of Pseudomonas . Bacteriological Reviews 33:419–443
    [Google Scholar]
  17. Holloway B. W., Richmond M. H. 1973; R factors used for genetic studies in strains of Pseudomonas aeruginosa and their origin. Genetical Research 21:103–105
    [Google Scholar]
  18. Ingram L. C. 1973; Deoxyribonucleic acid-deoxyribonucleic acid hybridization of R factors. Journal of Bacteriology 115:1130–1134
    [Google Scholar]
  19. Ingram L. C., Sykes R. B., Grinsted J., Saunders J. R., Richmond M. H. 1972; A transmissible resistance element from a strain of Pseudomonas aeruginosa containing no detectable extrachromosomal DNA. Journal of General Microbiology 72:269–279
    [Google Scholar]
  20. Isaac J. H., Holloway B. W. 1968; Control of pyrimidine biosynthesis in Pseudomonas aeruginosa . Journal of Bacteriology 96:1732–1741
    [Google Scholar]
  21. Kawakami Y., Mikoshiba F., Nagasaki S., Matsumoto H., Tazaki T. 1972; Prevalence of Pseudomonas aeruginosa strains possessing R factors in a hospital. Journal of Antibiotics 25:607–609
    [Google Scholar]
  22. Krishnapillai V. 1974; The use of bacteriophage in differentiating plasmids of Pseudomonas aeruginosa . Genetical Research (in the Press)
    [Google Scholar]
  23. Lowbury E. J. L., Kidson A., Lilly H. A., Ayliffe G. A., Jones R. J. 1969; Sensitivity of Pseudomonas aeruginosa to antibiotics: emergence of strains highly resistant to carbenicillin. Lancet ii:448–452
    [Google Scholar]
  24. Marvin D. A., Hohn B. 1969; Filamentous bacterial viruses. Bacteriological Reviews 33:172–209
    [Google Scholar]
  25. Matsumoto H., Tazaki T. 1973; FP5 factor, an undescribed sex factor of Pseudomonas aeruginosa . Japanese Journal of Microbiology 17:399–407
    [Google Scholar]
  26. Meynell E., Meynell G. G., Datta N. 1968; Phylogenetic relationships of drug-resistance factors and other transmissible bacterial plasmids. Bacteriological Reviews 32:55–83
    [Google Scholar]
  27. Morgan T., Saunders J. R., Stanisich V. A., Richmond M. H. 1973; Compatibility relationships between R factors originally isolated from strains of Pseudomonas aeruginosa . Proceedings of the Journal of General Microbiology 1:13–14
    [Google Scholar]
  28. Olsen R. H., Shipley P. 1973; Host range and properties of the Pseudomonas aeruginosa R factor R1822. Journal of Bacteriology 113:772–780
    [Google Scholar]
  29. Olsen R. H., Thomas D. D. 1973; Characteristics and purification of PRRi, an RNA phage specific for the broad host range Pseudomonas R1822 drug resistance plasmid. Virology 12:1560–1567
    [Google Scholar]
  30. Pemberton J. M. 1971 Conjugation in Pseudomonas aeruginosa Ph.D. thesis Monash University; Australia:
    [Google Scholar]
  31. Pemberton J. M., Holloway B. W. 1973; A new sex factor of Pseudomonas aeruginosa . Genetical Research 21:263–272
    [Google Scholar]
  32. Saunders J. R., Grinsted J. 1972; Properties of RP4, an R factor which originated in Pseudomonas aeruginosa s8. Journal of Bacteriology 112:690–696
    [Google Scholar]
  33. Stanisich V. A. 1974; Interaction between an R factor and an element conferring resistance to mercuric ions in Pseudomonas aeruginosa . Molecular and General Genetics 128:201–212
    [Google Scholar]
  34. Stanisich V. A., Holloway B. W. 1971; Chromosome transfer in Pseudomonas aeruginosa mediated by R factors. Genetical Research 17:169–172
    [Google Scholar]
  35. Stanisich V. A., Holloway B. W. 1972; A mutant sex factor of Pseudomonas aeruginosa . Genetical Research 19:91–108
    [Google Scholar]
  36. Wiman M., Bertani G., Kelly B., Sasaki I. 1970; Genetic map of Escherichia coli strain c. Molecular and General Genetics 107:1–31
    [Google Scholar]
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