Summary: Of 48 fungal isolates, 28 (including one Penicillium and three Aspergilli) secreted milk-clotting enzymes. This activity was in general related to the mycelial growth of a given isolate, but not with pH change of the culture filtrate during incubation. It was not found when mucilage was formed by isolates capable of doing so. The ratio of general proteolytic activity to milk-clotting activity varied from one organism to another, and with the age of culture of the same fungal strain.
Abdel-FattahA. F.,
MabroukS. S.,
El-HawwaryN. M.1972a; Distribution pattern of milk-clotting and proteolytic activities in some fungi. Acta biologica Academiae scientiarum hungaricae 23:55–60
Abdel-FattahA. F.,
MabroukS. S.,
El-HawwaryN. M.1972b; Production and some properties of rennin-like milk-clotting enzyme from Penicillium citrium
. Journal of General Microbiology 70:151–155
ArimaK.,
IwasakiS.,
TamuraG.1967; Milk-clotting enzyme from microorganisms. I. Screening test and the identification of the potent fungus. Agricultural and Biological Chemistry 31:540–545
BergkvistR.1963; The proteolytic enzymes of Aspergillus oryzae. I. Methods for the estimation and isolation of the proteolytic enzymes. Acta chemica scandinavica 17:1521–1540
LowryO. H.,
RosebroughN. J.,
FarrA. L.,
RandallR. J.1951; Protein measurements with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
SrinivasanR. A.,
IyengarM. K. K.,
BabbarI. J.,
ChakravortyS. C.,
DudaniA. T.,
IyaK. K.1964; Milk-clotting enzymes from micro-organisms. Applied Microbiology 12:475–478