The Fine Structure of and Spores with Special Reference to the Location of Crystal Antigen Free

Abstract

Summary: The ultrastructure of and spores has been examined using the freeze-etching technique. Labelled antibodies have been used in an attempt to locate crystal antigen in the spores of , which have a more complex structure than those of The results of morphological studies and the use of labelled antibodies to stain sections of sporulating cells of suggest a relationship between crystal protein and the sporecoat of the organism. The different aspects of ultrastructure and their relation to crystal structure and formation are discussed.

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1974-10-01
2024-03-28
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References

  1. Angus T. A. 1956; Association of toxicity with protein-crystalline inclusions in Bacillus sotto IsLiwata. Canadian Journal of Microbiology 2:416–425
    [Google Scholar]
  2. Beaman T. C., Pankratz H. S., Gerhardt P. 1971; Paracrystalline sheets reaggregated from solubilized exosporium of Bacillus cereus . Journal of Bacteriology 107:320–324
    [Google Scholar]
  3. de Barjac H., Bonnefoi A. 1962; Essai de classification biochemique et serologique de 24 souches de Bacillus de type thuringiensis. Entomophaga 7:5–17
    [Google Scholar]
  4. Delafield F. P., Somerville H. J., Rittenberg S. C. 1968; Immunological homology between crystal and spore protein of Bacillus thuringiensis . Journal of Bacteriology 96:713–720
    [Google Scholar]
  5. Ellar D. J., Lundgren D. G. 1966; Fine structure of sporulation in Bacillus cereus grown in chemically defined medium. Journal of Bacteriology 92:1748–1764
    [Google Scholar]
  6. Ellis D. A. 1961; A new universal buffer. Nature, London 192:1099
    [Google Scholar]
  7. Fitz-James P. C. 1962; The initial formation of parasporal inclusions in bacilli. Proceedings V International Congress of Electron Microscopy 2:RR. 10
    [Google Scholar]
  8. Fitz-James P. C. 1965; Spore formation in wild and mutant strains of Bacillus cereus and some effects of inhibitors. In Mecanismes de Regulation des Activités Cellulaires chez les Micro-organismes p. 529 Edited by Senez M. J. C. Paris: Centre Nationale de la Recherché Scientifique;
    [Google Scholar]
  9. Gerhardt P., Black S. H. 1961; Permeability of bacterial spores. II. Molecular variables affecting solute permeation. Journal of Bacteriology 82:750–760
    [Google Scholar]
  10. Gerhardt P., Ribi E. 1964; Ultrastructure of the exosporium enveloping spores of Bacillus cereus . Journal of Bacteriology 88:1774–1789
    [Google Scholar]
  11. Granboulan P., Leduc E. G. 1967; Ultrastructural cytochemistry of Bacillus subtilis . Journal of Ultrastructure Research 20:111–126
    [Google Scholar]
  12. Hannay C. L., Fitz-James P. C. 1955; The protein crystals of Bacillus thuringiensis Berliner. Canadian Journal of Microbiology 1:694–710
    [Google Scholar]
  13. Heimpel A. M., Angus T. A. 1958; The taxonomy of insect pathogens related to Bacillus cereus . Canadian Journal of Microbiology 4:531–542
    [Google Scholar]
  14. Holmes K. C., Monro R. E. 1965; Studies on the structure of parasporal inclusions from Bacillus thuringiensis . Journal of Molecular Biology 14:572–581
    [Google Scholar]
  15. Holt S. C., Leadbetter E. R. 1969; Comparative ultrastructure of selected aerobic sporeforming bacteria: a freeze etching study. Bacteriological Reviews 33:346–378
    [Google Scholar]
  16. Labaw L. W. 1964; The structure of Bacillus thuringiensis Berliner crystals. Journal of Ultrastructure Research 10:66–75
    [Google Scholar]
  17. Leadbetter E. R., Holt S. C. 1968; The fine structure of Bacillus fastidiosus . Journal of General Microbiology 52:299–308
    [Google Scholar]
  18. Lecadet M. M., Chevrier G., Dedonder R. 1972; Analysis of a protein fraction in the sporecoats of Bacillus thuringiensis . European Journal of Biochemistry 25:349–358
    [Google Scholar]
  19. Moor H., Muhlethaler K., Waldner H., Frey-Wyssling A. 1961; A new freezing ultramicrotome. Journal of Biophysical and Biochemical Cytology 10:1–14
    [Google Scholar]
  20. Moriarty G. C., Halmi N. S. 1972; Electron microscopic study of the adrenocorticotropin producing cell with the use of the unlabelled antibody and the soluble peroxidase–antiperoxidase complex. Journal of Histochemistry and Cytochemistry 20:590–603
    [Google Scholar]
  21. Norris J. R. 1969; Macromolecule synthesis during sporulation of Bacillus thuringiensis . Spores IV p. 45 Edited by Campbell L. L. Washington D.C.: American Society for Microbiology;
    [Google Scholar]
  22. Norris J. R. 1971; The protein crystal of Bacillus thuringiensis biosynthesis and physical structure. Microbial Control of Insects and Mites p. 229 Edited by Burges H. D., Hussey N. W. London: Academic Press;
    [Google Scholar]
  23. Short J., Walker P. D. 1971; Structure of bacterial spores as revealed by freeze etching. Spore Research (1971) p. 189 Edited by Barker A. N., Gould G. W., Wolf J. London: Academic Press;
    [Google Scholar]
  24. Singer S. J., Schick A. F. 1961; The properties of specific stains for electron microscopy prepared by the conjugation of antibody molecules with ferritin. Journal of Biophysical and Biochemical Cytology 9:519–537
    [Google Scholar]
  25. Somerville H. J. 1971; Formation of the parasporal inclusion of Bacillus thuringiensis . European Journal of Biochemistry 18:226–237
    [Google Scholar]
  26. Somerville H. J., Delafield F. P., Rittenberg S. C. 1968; Biochemical homology between crystal and spore protein of Bacillus thuringiensis . Journal of Bacteriology 96:721–731
    [Google Scholar]
  27. Somerville H. J., Delafield F. P., Rittenberg S. C. 1970; Urea-mercaptoethanol-soluble protein from spores of Bacillus thuringiensis and pther species. Journal of Bacteriology 101:551–560
    [Google Scholar]
  28. Somerville H. J., James C. R. 1970; Association of the crystalline inclusion of Bacillus thuringiensis with the exosporium. Journal of Bacteriology 102:580–583
    [Google Scholar]
  29. Somerville H. J., James C. R., Ruffell R. N., Norris J. R. 1971; The parasporal inclusion of Bacillus finitimus . In Spore Research (1971) p. 193 Edited by Barker A. N., Gould G. W., Wolf J. London: Academic Press;
    [Google Scholar]
  30. Somerville H. J., Jones M. L. 1972; DNA competition studies within the Bacillus cereus group of bacilli. Journal of General Microbiology 73:257–265
    [Google Scholar]
  31. Sternberger L. A., Hardy P. H. Jun., Cuculis J. J., Meyer H. G. 1970; The unlabelled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen–antibody complex (horseradish peroxidase–antihorseradish peroxidase) and its use in identification of spirochetes. Journal of Histochemistry and Cytochemistry 18:315–333
    [Google Scholar]
  32. Walker P. D., Baillie A., Thomson R. O., Batty I. 1966; The use of ferritin labelled antibodies in the location of spore and vegetative antigens of Bacillus cereus . The Journal of Applied Bacteriology 29:512–518
    [Google Scholar]
  33. Young I. E., Fitz-James P. C. 1959; Chemical and morphological studies of bacterial spore formation. II. Spore and parasporal protein formation in Bacillus cereus var. alesti. Journal of Biophysical and Biochemical Cytology 6:483–498
    [Google Scholar]
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