1887

Abstract

Summary: Germination and outgrowth of spores of occurred rapidly and synchronously in a medium containing casein hydrolysate, vitamins and metal salts. Germination began immediately after inoculation and within 10 min more than 90% of the spores were phase dark. Swelling of the spores began immediately thereafter and by 25 min after inoculation more than 70% of the spores were swollen. Elongation of the swollen spores was first detected at about 35 min; by 65 min 80% of the spores had become cells, and by 90 min cell division had started. Increases in turbidity, RNA and protein were first detected during swelling and an increase in DNA was first observed at the beginning of elongation. By the time cell division began DNA had doubled. When spores were heated at 80°C for 10 min, 20% survived; the survivors germinated very much more slowly than unheated spores and only formed cells if anaerobic conditions were maintained. The increases in RNA, protein and DNA occurred much later during outgrowth than with unheated spores.

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1974-10-01
2021-10-23
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References

  1. Anthony G., Guest J. R. 1968; Deferred metabolism of glucose by Clostridium tetanomorphum . Journal of General Microbiology 154:277–286
    [Google Scholar]
  2. Burton K. 1956; A study of the conditions and mechanisms of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochemical Journal 62:315–323
    [Google Scholar]
  3. Cassier M., Sebald M. 1969; Germination lysozyme-dépendante des spores de Clostridium perfringens atcc 3624 après traitement thermique. Annales de I’Institut Pasteur 117:312–324
    [Google Scholar]
  4. Church B. D., Germaine G. R., Rowley D. B. 1971; Mode of radiation resistance of Clostridium botulinum spores. Government Reports Announcements (U.S.) 71:461–50
    [Google Scholar]
  5. Donnellan J. E. Jun., Nags E. H., Levinson H. S. 1965; Nucleic acid synthesis during germination and postgerminative development. In Spores vol. 3 pp. 152–161 Edited by Campbell L. L., Halvorson H. O. Ann Arbor, Michigan: American Society for Microbiology;
    [Google Scholar]
  6. Duncan C. L., Labbe R. G., Reich R. R. 1972; Germination of heat- and alkali-altered spores of Clostridium perfringens type A by lysozyme and an initiation protein. Journal of Bacteriology 109:550–559
    [Google Scholar]
  7. Fitz-James P. C. 1955; The phosphorus fractions of Bacillus cereus and Bacillus megaterium. II. A correlation of the chemical with the cytological changes occurring during spore germination. Canadian Journal of Microbiology 1:525–548
    [Google Scholar]
  8. Fujioka R. S., Frank H. A. 1966; Nutritional requirement for germination, outgrowth and vegetative growth of putrefactive anaerobe 3679 in a chemically defined medium. Journal of Bacteriology 92:1515–1520
    [Google Scholar]
  9. Gould G. W., Hitchins A. D. 1963; Sensitization of bacterial spores to lysozyme and to hydrogen peroxide with agents which rupture disulphide bonds. Journal of General Microbiology 33:413–423
    [Google Scholar]
  10. Hirsch A., Grinsted E. 1954; Methods for the growth and enumeration of anaerobic spore-formers from cheese, with observations on the effect of nisin. Journal of Dairy Research 21:101–110
    [Google Scholar]
  11. Levinson H. S., Hyatt M. T. 1970; Effects of temperature on activation, germination and outgrowth of Bacillus megaterium spores. Journal of Bacteriology 101:58–64
    [Google Scholar]
  12. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  13. Mackey B. M., Morris J. G. 1972; Calcium dipicolinate-provoked germination and outgrowth of spores of Clostridium pasteurianum . Journal of General Microbiology 73:315–324
    [Google Scholar]
  14. Mead G. C. 1971; The amino acid-fermenting Clostridia. Journal of General Microbiology 67:47–56
    [Google Scholar]
  15. O’Brien R. W., Morris J. G. 1971; Oxygen and the growth and metabolism of Clostridium acetobutylicum . Journal of General Microbiology 68:307–318
    [Google Scholar]
  16. Rowley D. B., El-Bisi H. M., Anellis A., Snyder O. P. 1970; Resistance of Clostridium botulinum spores to ionising radiation as related to radappertization of foods. In Proceedings of the First U.S. – Japan Conference on Toxic Micro-organisms pp. 459–467 Edited by Herzberg M. Washington D.C.: U.S. Government Printing Office;
    [Google Scholar]
  17. Schneider W. C. 1957; Determination of nucleic acid in tissues by pentose assay. In Methods in Enzymology vol. 3 pp. 680–684 Edited by Colowick S. P., Kaplan N. O. New York and London: Academic Press;
    [Google Scholar]
  18. Sebald M., Ionesco H. 1972; Germination lzP-dépendante des spores de Clostridium botulinum type E. Comptes rendus hebdomadaire des séances de l’Académie des science 275:2175–2177
    [Google Scholar]
  19. Steinberg W., Halvorson H. O. 1968; Timing of enzyme synthesis during outgrowth of spores of Bacillus cereus. II. Relationship between ordered enzyme synthesis and deoxyribonucleic acid replication. Journal of Bacteriology 95:479–489
    [Google Scholar]
  20. Steinberg W., Idriss J., Rodenberg S., Halvorson H. O. 1969; Developmental changes accompanying the breaking of the dormant state in bacteria. In Dormancy and Survival pp. 11–49 Edited by Woolhouse H. W. Cambridge University Press;
    [Google Scholar]
  21. Strange R. E., Hunter J. R. 1969; Outgrowth and the synthesis of macromolecules. In The Bacterial Spore pp. 445–483 Edited by Gould G. W., Hurst A. London and New York: Academic Press;
    [Google Scholar]
  22. Waites W. M., Wyatt L. R. 1971; Germination of spores of Clostridium bifermentans by certain amino acids, lactate and pyruvate in the presence of sodium or potassium ions. Journal of General Microbiology 67:215–222
    [Google Scholar]
  23. Waites W. M., Wyatt L. R. 1974; The effect of pH, germinants and temperature on the germination of spores of Clostridium bifermentans . Journal of General Microbiology 80:253–258
    [Google Scholar]
  24. Wyatt L. R., Waites W. M. 1971; Studies with spores of Clostridium bifermentans; comparison of germination mutants. In Spore Research – 1971 pp. 123–132 Edited by Barker A. N., Gould G. W., Wolf J. London and New York: Academic Press;
    [Google Scholar]
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