1887

Abstract

SUMMARY: absorbed -methionine with a value of 25μ, optimum temperature 42 to 45°C. -Ethionine, S-methyl--cysteine, S-ethyl--cysteine and α-methyl--methionine inhibited methionine transport competitively, with values of 0·10, 0·35, 0·40 and 0·20 m respectively.

Using lysozyme, EDTA and trypsin, membrane vesicles were prepared from cells grown with methionine. Methionine accumulation in the vesicles was enhanced both by ascorbate-phenazine methosulphate and by several physiological electron donors; the most effective were FAD+malate, malate, succinate or 2-oxoglutarate. Oxidation of succinate, but not of malate, was inhibited by malonate; oxidation of malate was inhibited by azide; FAD did not reverse azide inhibition.

Tracer methionine was recovered from the membranes both as the unchanged amino acid and methionine sulphoxide.

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1974-09-01
2024-12-10
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References

  1. Arrigoni O., Singer T. P. 1962; Limitations of the phenazine methosulphate assay for succinic and related dehydrogenases. Nature, London 193:1256–1258
    [Google Scholar]
  2. Ayling P. D., Bridgeland E. S. 1972; Methionine transport in wild-type and transport-defective mutants of Salmonella typhimurium . Journal of General Microbiology 73:127–141
    [Google Scholar]
  3. Britten R. J., McClure F. T. 1962; The amino acid pool in Escherichia coli . Bacteriological Reviews 26:292–335
    [Google Scholar]
  4. Cohen G. N., Monod J. 1957; Bacterial permeases. Bacteriological Reviews 21:169–194
    [Google Scholar]
  5. Gits J. J., Grenson M. 1967; Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. III. Evidence for a specific methionine-transporting system. Biochimica et biophysica acta 135:507–516
    [Google Scholar]
  6. Gryder R. M., Adams E. 1970; Properties of the inducible hydroxy proline transport system of Pseudomonas putida . Journal of Bacteriology 101:948–958
    [Google Scholar]
  7. Hunter D. R., Segel I. H. 1973; Control of the general amino acid permease of Penicillium chrysogenum by transinhibition and turnover. Archives of Biochemistry and Biophysics 154:387–399
    [Google Scholar]
  8. Kaback H. R. 1971; Bacterial membranes. In Methods in Enzymology vol. 22 pp. 99–120 Edited by Jacoby W. B. New York and London: Academic Press;
    [Google Scholar]
  9. Kaback H. R., Milner L. S. 1970; Relationship of a membrane-bound d-(–)-lactic dehydrogenase to amino acid transport in isolated bacterial membrane preparations. Proceedings of the National Academy of Sciences of the United States of America 66:1008–1015
    [Google Scholar]
  10. Kadner R. J. 1974; Transport systems for L-methionine in Escherichia coli . Journal of Bacteriology 117:232–241
    [Google Scholar]
  11. Kay W. W., Gronlund A. F. 1969a; Amino acid transport in Pseudomonas aeruginosa . Journal of Bacteriology 97:273–281
    [Google Scholar]
  12. Kay W. W., Gronlund A. F. 1969b; Amino acid pool formation in Pseudomonas aeruginosa . Journal of Bacteriology 100:282–291
    [Google Scholar]
  13. Kepes A., Cohen G. N. 1962; Permeation. In The Bacteria vol. 4 pp. 179–221 Edited by Gunsalus J. C. New York: Academic Press;
    [Google Scholar]
  14. Lombardi F. J., Kaback H. R. 1972; Mechanisms of active transport in isolated bacterial membrane vesicles. VIII. The transport of amino acids by membranes prepared from Escherichia coli . Journal of Biological Chemistry 247:7844–7857
    [Google Scholar]
  15. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  16. Mäntsälä P. 1974; Correlations between pantothenate uptake, phospholipid synthesis and pantothenate-binding protein formation in Pseudomonas fluorescens p-2. Acta chemica scandinavica 28:78–84
    [Google Scholar]
  17. Matin A., Konings W. N. 1973; Transport of lactate and succinate by membrane vesicles of Escherichia coli, Bacillus subtilis and a Pseudomonas species. European Journal of Biochemistry 34:58–67
    [Google Scholar]
  18. Miller D. L., Rodwell V. W. 1971a; Metabolism of basic amino acids in Pseudomonas putida. Properties of the inducible lysine transport system. Journal of Biological Chemistry 246:1765–1771
    [Google Scholar]
  19. Miller D. L., Rodwell V. W. 1971b; Metabolism of basic amino acids in Pseudomonas putida. Intermediates in l-arginine catabolism. Journal of Biological Chemistry 246:5053–5058
    [Google Scholar]
  20. Piperno J. R., Oxender D. L. 1968; Amino acid transport systems in Escherichia coli k-12. Journal of Biological Chemistry 243:5914–5920
    [Google Scholar]
  21. Rosenfeld H., Feigelson P. 1969; Synergistic and product induction of the enzymes of tryptophan metabolism in Pseudomonas acidovorans . Journal of Bacteriology 97:697–704
    [Google Scholar]
  22. Segal W., Starkey R. L. 1969; Microbial decomposition of methionine and identity of the resulting sulfur products. Journal of Bacteriology 98:908–913
    [Google Scholar]
  23. Short S. A., White D. C., Kaback H. R. 1972; Active transport in isolated bacterial membrane vesicles. V. The transport of amino acids by membrane vesicles prepared from Staphylococcus aureus . Journal of Biological Chemistry 247:298–304
    [Google Scholar]
  24. Sprott G. D., MacLeod R. A. 1972; Na+-dependent amino acid transport in isolated membrane vesicles of a marine pseudomonad energized by electron donors. Biochemical and Biophysical Research Communications 47:838–845
    [Google Scholar]
  25. Stinnett J. D., Guyman L. F., Eagon R. G. 1973; A novel technique for the preparation of transport-active membrane vesicles from Pseudomonas aeruginosa: observations of gluconate transport. Biochemical and Biophysical Research Communications 52:284–290
    [Google Scholar]
  26. Veeger C., der Vartanian D. V., Zeylemaker W. P. 1969; Succinate dehydrogenase. In Methods in Enzymology vol. 13 pp. 81–90 Edited by Lowenstein J. M. New York and London: Academic Press;
    [Google Scholar]
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