1887

Abstract

SUMMARY: Starvation of baker’s yeast, , in 2% glycerol induces high levels of petite mutants. The rate of mutation is highest when exponential phase cells are used to inoculate the starvation medium. The starved cells are exceedingly sensitive to ethidium bromide mutagenesis. Cycloheximide and sodium nalidixate enhance starvation-induced mutation (although nalidixate reduces the ethidium bromide induction). Starvation-induced mutation is inhibited by rifampicin or erythromycin. The appearance of petite mutants is preceded by a fall in both o and mitochondrial DNA.

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1974-09-01
2024-04-19
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References

  1. Ezekiel D. H., Hutchins J. E. 1968; Mutations affecting RNA polymerase associated with rifampicin resistance in Escherichia coli . Nature, London 220:276–277
    [Google Scholar]
  2. Hollenberg C. P., Borst P. 1971; Conditions that prevent p induction by ethidium bromide. Biochemical and Biophysical Research Communications 45:1250–1254
    [Google Scholar]
  3. Luha A. A., Sarcoe L. E., Whittaker P. A. 1971; Biosynthesis of yeast mitochondria. Drug effects on the petite negative yeast Kluyveromyces lactis . Biochemical and Biophysical Research Communications 44:396–402
    [Google Scholar]
  4. Mounolou J. C. 1967; Role d'un ADN specifique dans le déterminisme genétique et physiologique des mito-chondries de la levure . D.EsSc. thesis Faculte des Sciences de Paris; France: pp. 86–89
    [Google Scholar]
  5. Mounolou J. C., Jakob H., Slonimski P. P. 1966; Mitochondrial DNA from yeast petite mutants: specific changes of buoyant density corresponding to different cytoplasmic mutations. Biochemical and Biophysical Research Communications 24:218–224
    [Google Scholar]
  6. Moustacchi E., Williamson D. H. 1966; Physiological variations in satellite components of yeast DNA detected by density gradient centrifugation. Biochemical and Biophysical Research Communications 23:56–61
    [Google Scholar]
  7. Nagai S. 1969; High-frequency production of respiratory mutants in yeast under nutritional deficiencies. Mutation Research 8:557–564
    [Google Scholar]
  8. Ogur M., St. John R., Nagai S. 1957; Tetrazolium overlay technique for population studies of respiratory deficiency in yeast. Science, New York 125:928–929
    [Google Scholar]
  9. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. Journal of Molecular Biology 4:430–433
    [Google Scholar]
  10. Sueoka N. 1961; Variation and heterogeneity of base composition of deoxyribonucleic acids: a compilation of old and new data. Journal of Molecular Biology 3:31–40
    [Google Scholar]
  11. Vidova M., Kovac L. 1972; Nalidixic acid prevents the induction of yeast cytoplasmic respiration deficient mutants by intercalating drugs. Federation of European Biochemical Societies Letters 22:347–351
    [Google Scholar]
  12. Wallis O. C., Ottolenghi P., Whittaker P. A. 1972; Induction of petite mutants in yeast by starvation in glycerol. Biochemical Journal 127:46–47 p.
    [Google Scholar]
  13. Whittaker P. A., Hammond R. C., Luha A. A. 1972; Mechanism of mitochondrial mutation in yeast. Nature New Biology 238:266–268
    [Google Scholar]
  14. Whittaker P. A., Wallis O. C. 1971; Biosynthesis of yeast mitochondria. Effects of rifampicin. Biochemical Journal 125:82 p.
    [Google Scholar]
  15. Whittaker P. A., Wright M. 1972; Prevention by cycloheximide of petite mutation in yeast. Biochemical and Biophysical Research Communications 48:1455–1459
    [Google Scholar]
  16. Williamson D. H. 1970; The effect of enviromental and genetic factors on the replication of mitochondrial DNA in yeast. Symposia of the Society for Experimental Biology 24:247–276
    [Google Scholar]
  17. Williamson D. H., Maroudas N. G., Wilkie D. 1971; Induction of the cytoplasmic petite mutation in Saccharomyces cerevisiae by the antibacterial antibiotics erythromycin and chloramphenicol. Molecular and General Genetics 111:209–223
    [Google Scholar]
  18. Williamson D. H., Moustacchi E., Fennell D. 1971; A procedure for rapidly extracting and estimating the nuclear and cytoplasmic DNA components of yeast cells. Biochimica et biophysica acta 238:369–374
    [Google Scholar]
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