1887

Abstract

SUMMARY: has been shown to produce a capsule which can be visualized by electron microscopy following staining with ruthenium red. The capsule of possesses no obvious structure and extends for 17 to 24 nm beyond the cytoplasmic membrane. Ruthenium red also stains the capsule of var. so that it can be seen by electron microscopy. Five other bovine mycoplasmas, var. and a T-mycoplasma, and one human mycoplasma, were found not to produce a capsule.

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/content/journal/micro/10.1099/00221287-83-2-393
1974-08-01
2024-04-18
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References

  1. Andrews B. E., Leach R. H., Gourlay R. N., Howard C. J. 1973; Enhanced isolation of Mycoplasma dispar by substitution of ampicillin for benzylpenicillin in growth media. Veterinary Record 93:603
    [Google Scholar]
  2. Buttery S. H., Plackett P. 1960; A specific polysaccharide from Mycoplasma mycoides . Journal of General Microbiology 23:357–368
    [Google Scholar]
  3. Domermuth C. H., Nielsen M. H., Freundt E. A., Bfrch-Andersen A. 1964; Ultrastructure of Mycoplasma species. Journal of Bacteriology 88:727–744
    [Google Scholar]
  4. Glynn A. A. 1972; Bacterial factors inhibiting host defence mechanisms. Symposia of the Society for General Microbiology 22:75–112
    [Google Scholar]
  5. Gourlay R. N., Leach R. H. 1970; A new mycoplasma species isolated from pneumonic lungs of calves (Mycoplasma dispar sp. nov.). Journal of Medical Microbiology 3:111–123
    [Google Scholar]
  6. Gourlay R. N., Thrower K. J. 1968; Morphology of Mycoplasma mycoides thread-phase growth. Journal of General Microbiology 54:155–159
    [Google Scholar]
  7. Green F., Hanson R. P. 1973; Ultrastructurc and capsule of Mycoplasma meleagridis . Journal of Bacteriology 116:1011–1018
    [Google Scholar]
  8. Howard C. J., Gourlay R. N. 1973a; Inhibition by normal rabbit sera of the growth of T-mycoplasma strains isolated from different animal spec ies. Journal of General Microbiology 78:277–285
    [Google Scholar]
  9. Howard C. J., Gourlay R. N. 1973b; Serological comparison of bovine T-mycoplasmas. Journal of General Microbiology 79:129–134
    [Google Scholar]
  10. Langer P. H., Carmichael L. E. 1963; Identification of pneumoenteritis isolates from cattle as mycoplasma. In Proceedings of the 67th Meeting of the U.S . Livestock Sanitary Association pp 129–137
    [Google Scholar]
  11. Luft J. H. 1971; Ruthenium red and violet. I. Chemistry, purification, methods of use for electron microscopy and mechanism of action. Anatomical Record 171:347–368
    [Google Scholar]
  12. MacLeod C. M. 1958; Pathogenic properties of bacteria and defence mechanisms of the host. In Bacterial and Mycotic Infections of Man, 3rd edn. pp 83–113 Dubos R. J. Edited by London: Pitman Medical;
    [Google Scholar]
  13. Meynell G. G. 1961; Phenotypic variation and bacterial infection. Symposia of the Society for General Microbiology 11:174–195
    [Google Scholar]
  14. Millonig G. 1961; Advantage of a phosphate buffer for osmium tetroxide solutions in fixation. Journal of Applied Physics 32:1637
    [Google Scholar]
  15. Sabatini D. D., Bensch K., Barrnett R. J. 1963; Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. Journal of Cell Biology 17:19–58
    [Google Scholar]
  16. Springer E. L., Roth I. L. 1973; The ultrastructure of the capsules of Diplococcus pneumoniae and Klebsiella pneumoniae stained with ruthenium red. Journal of General Microbiology 74:21–31
    [Google Scholar]
  17. Subcommiitee On the Taxonomy of Mycopl Asmatale 1972; Proposal for minimal standards for description of new species of the order Mycoplasmatales . International Journal of Systematic Bacteriology 22184–188
    [Google Scholar]
  18. Venable J. H., Coggeshall R. 1965; A simplified lead citrate stain for use in electron microscopy. Journal of Cell Biology 25:407–408
    [Google Scholar]
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