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Abstract
SUMMARY: mutant of Pseudomonas aeruginosa affected in aerobic growth (aer) was partially characterized. When shifted from anaerobic to aerobic conditions mutant bacteria continued growth. During this aerobic growth after the shift the mutant bacteria differed from the wild type in some respects. First, the concentration of intracellular haem decreased gradually, whereas coproporphyrin was released into the culture fluid. Apparently haem synthesis is blocked under aerobic conditions, so oxygen uptake by the mutant culture did not increase as much as by the wild type after a shift. Second, the anaerobic nitrate-respiratory system of the mutant exhibited an altered sensitivity to oxygen after a shift to aerobiosis. Nitrate and nitrite reduction were not inhibited and nitrite reductase synthesis was not repressed by oxygen. P/O values determined in intact bacteria showed that respiration with oxygen was still coupled to ATP formation in the mutant.
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